摘要
在单因素试验基础上,采用响应面法的(Box-Benhnken)组合设计原理,对淫羊藿总黄酮的提取工艺参数进行优化。淫羊藿总黄酮的最佳提取工艺条件为:提取时间1.73 h、水料比31∶1(mL/g),温度67.5℃、乙醇浓度79%,淫藿黄酮类化合物的平均提取率为2.054%,比理论预测值高0.08%。
On the basis of single factor experiment,the conditions for extracting flavonoids from Herba Epimedii were optimized with response surface method (RSM). The optimal conditions for flavones extraction were obtained as follows: extraction temperature of 67.5℃ ,water/solid ratio of 31, extraction time of 1.73 h,and ethanol concentration of 79%. Under the above conditions,the one-time extraction rate was up to 2.054%,which was 0.08% more than the theoretical value.
出处
《中兽医医药杂志》
2012年第6期16-19,共4页
Journal of Traditional Chinese Veterinary Medicine
基金
甘肃省教育厅研究生导师资助项目(编号1009-09)
甘肃省高校河西走廊特色资源利用省级重点实验室科研资助项目(编号XZ1008)
关键词
淫羊藿
总黄酮
提取
响应面分析
Herba Epimedii
flavonoids
extraction
response surface experiment
作者简介
林敏(1967-),女,副教授,主要从事天然产物有效成分提取与分析,E—mail:linmin0936@163.com