摘要
目的:观察绞股蓝总皂苷含药血清对光老化HaCaT细胞p38促分裂原活化蛋白激酶(p38MAPK)蛋白和Jun基因(c-Jun)mRNA表达的影响,探讨绞股蓝总皂苷含药血清抗皮肤光老化的作用机制。方法:采用中波紫外线(UVB)照射建立光老化HaCaT细胞模型,加低、中、高剂量绞股蓝总皂苷含药血清于光老化HaCaT细胞模型培养液中培养,并设空白对照组、空白血清组和UVB模型组。采用Western blotting和RT-PCR方法检测各组细胞p38MAPK蛋白和c-Jun mRNA的表达水平。结果:与空白对照组比较,UVB模型组细胞p38MAPK蛋白和c-Jun mRNA表达显著上调,差异有统计学意义(P<0.01);与UVB模型组比较,低、中和高剂量含药血清组细胞p38MAPK蛋白和c-Jun mRNA表达显著下调,差异有统计学意义(P<0.01);与空白血清组比较,低、中和高剂量含药血清组细胞p38MAPK蛋白和c-Jun mRNA表达下降(P<0.01)。结论:绞股蓝总皂苷含药血清可能通过抑制p38MAPK蛋白和c-Jun mRNA表达抑制皮肤光老化。
Objective To observe the effect of gypenosides-containing serum on the expressions of p38 mitogen-activated protein kinase（p38MAPK）protein and c-Jun mRNA in photoaging HaCaT cells and to explore the mechanism of inhibitory effect of gypenosides-containing serum on photoaging.Methods The photoaging HaCaT cell model was established by UVB radiation,low,middle,and high doses of gypenosides-containing serum were used in the culture fluid of phatoaging HaCaT cell models;meanwhile blank control group,blank serum group and UVB model group were set up.The expression levels of p38MAPK protein and c-Jun mRNA of cells in each group were measured by Western blotting and RT-PCR methods.Results Compared with blank control group,the expression levels of p38MAPK protein and c-Jun mRNA of the cells in UVB model group were significantly increased,the difference had statistical significance（P0.01）.Compared with UVB model group,the expression levels of p38MAPK protein and c-Jun mRNA of cells in low,middle and high doses of gypenosides-containing serum groups were significantly decreased,the difference had statistical significance（P0.01）.Compared with blank serum group,the expression levels of p38 MAPK protein and c-Jun mRNA of the cells in low,middle and high doses of gypensides-containing serum groups were decreased（P0.01）.Conclusion Gypenosides-containing serum could prevent the skin photoaging by inhibinting the expressions of p38MAPK protein and c-Jun mRNA.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2012年第6期1147-1151,共5页
Journal of Jilin University：Medicine Edition
基金
辽宁省科技厅自然科学基金资助课题(20102149)
作者简介
陶冶（1984-），女，辽宁省丹东市人，在读医学博士，主要从事中医美容学方面的研究。
[通信作者]吴景东（Tel：024—31207258，E-mail：lnzywjd0719@163．com）
