摘要
目的观察木犀草素对小鼠气道平滑肌细胞增殖的影响及其与PI3 K/AKT通路的关系。方法分离并培养小鼠气道平滑肌细胞,α-actin免疫细胞化学染色鉴定,细胞分4组:对照组、PDGF组、PDGF+LUT组、LUT组,MTT法检测细胞增殖,Western Blot方法观察p-AKT表达的变化。结果 MTT检测发现,PDGF刺激气道平滑肌细胞后A490值较对照组明显增加(P<0.01),PDGF+LUT组A490值较PDGF组明显降低(P<0.05),LUT组A490值较对照组无明显变化;Western Blot发现,对照组p-AKT极低,PDGF组明显升高,PDGF+LUT组p-AKT较PDGF组降低。结论木犀草素能够通过抑制AKT的磷酸化水平,限制体外培养的气道平滑肌细胞的PI3 K/AKT通路激活,从而发挥抑制小鼠气道平滑肌增殖的作用。
Objective It is to investigate the effect of luteolin on proliferation of airway smooth muscle cells（ASMC） in mice and its relationship with PI3 K/AKT signal pathway.Methods ASMC of the mice were isolated and cultured.Then the cells were identified by α-actin immuncytochemistry and divided into 4 groups： control group,PDGF group,PDGF＋LUT group and LUT group.The proliferation of ASMC was detected by Methylthiazolyldiphenyl-Tetrazolium bromide assay,and the level phosphorylation of AKT was detected by Western blot.Results MTT detection showed that A490 value increased obviously compared with control group（P0.01） after the ASMC were simulated by PDGF.The value of PDGF＋LUT group was significantly lower than that of PDGF group,There was no significant difference in the value between LUT group and the control group.Western Blot detection showed that p-AKT was much lower in the control group,and that increased obviously in PDGF group,which was higher than that in PDGF＋LUT group.Conclusion Luteolin may inhibit phosphorylation level of AKT to inhibit the activation of PI3 K/Akt signal pathway of ASMCs,thus to inhibit the proliferation of these cells in the mice.
出处
《现代中西医结合杂志》
CAS
2012年第35期3896-3898,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
作者简介
[作者简介]李若然（1974-），男，医学博士，主治医师，主要从事呼吸疾病诊治工作。
