摘要
蔗将硅转运蛋白基因OsLsi1和OsLsi2转入美女樱中,研究该基因对美女樱抗寒性的影响。对经PCR鉴定的阳性转基因美女樱植株进行RT-PCR检测,结果表明转运蛋白基因OsLsi1和OsLsi2在转基因美女樱中能够正常转录并表达,说明这两个基因已经整合到美女樱中;在0℃低温下进行0、4d和7d低温处理,测定丙二醛(MDA)含量、脯氨酸含量、超氧化物歧化酶(SOD)活性和过氧化物酶(POD),结果发现,转基因植株的抗寒性比非转基因的高,且转OsLsi1基因植株比OsLsi2基因抗寒能力强,为创建美女樱抗寒种质资源奠定了基础。
The Silicon transporter protein OsLsi1 and OsLsi2 gene were transformed into Verbena nybrida to study the impacts on Verbena nybrida.The PCR results showed that the Silicon transporter protein OsLsi1 and OsLsi2 gene were successfully transferred into Verbena nybrida and the Normal transcription and expression of the Silicon transporter protein OsLsi1 and OsLsi2 gene in transgenic Verbena nybrida were indentified by RT-PCR.The MDA（malondialdehyde）,proline content,and SOD,POD activity of Verbena nybrida were respectively tested on 0,4 d,7 d under the condition of 0℃ treatment.These results strongly suggested that the cold resistance of the transgenic Verbena nybrida was higher than non-transgenic Verbena nybrida.,and the cold tolerance of OsLsi1 gene transformed plants higher than the plants transformed with OsLsi2,which provided freezing-resistance germplasm of Verbena nybrida.,to some extent.
出处
《分子植物育种》
CAS
CSCD
北大核心
2012年第6期740-746,共7页
Molecular Plant Breeding
基金
黑龙江省研究生创新科研项目(YJSCX2011-072HLJ)资助
作者简介
通讯作者,fan_xuer2000@yahoo．com．cn
