摘要
通过几何学的方法,就如何校正旋光仪的零点、确定旋光方向、测定旋光度以及计算比旋光度进行了探讨。首先根据三分视场的明暗变化确定平面偏振光的零点位置,结合旋光仪刻度盘的读数求算出旋光仪的零点校正值。根据平面偏振光的旋光方向与旋光仪的刻度盘旋转方向之间的几何关系,判断旋光仪的刻度盘旋转方向是否与平面偏振光的旋光方向一致,据此判断蔗糖及其水解产物的旋光方向,再根据旋光仪刻度盘旋转的角度求算出平面偏振光旋转的角度,进而求算出相应的比旋光度。结果表明,根据平面偏振光的旋光方向与旋光仪的刻度盘旋转方向之间的几何关系,可以正确确定旋光物质的旋光方向和求算其旋光度。
By the method of geometry, the correction of the zero of tile imlarimeter, the determination of the rotatory direction, anti the measurement of the optical rotation and the calculation of the specific rotatory power were discussed. According to light and shade change of three region field of view, the zero position of the plane polarized light was determined. By t,sing /he polarimeter dial readings, the measurement and calculation of the polarimeter zero correction were made. According to the geometric relationship hetween tire rotatory direction of plane polarized light and direction of rotation of the polarimeter dial, judgment was made whether the direction of rotation of the polarimeter dial was consistent with the rotatory direction of plane polarized light, pursuant to which the judgmenl for the rotatory direction of the sucrose and its hydrolysis product were made. And then, in accordance with polarimeter dial rotating angle the angle of rotation of plane polarized light was calculated. Thus the corresponding specific rotatory power was ohtained. The resuhs show that according to the geometrie relationship between the rotator direction of plane polarized light and direction of rotation of the polarimeter dial, the rotator direction of optically active subslanee can be correctly determined, and along with their optical rotation calculation.
出处
《实验室研究与探索》
CAS
北大核心
2012年第10期22-25,137,共5页
Research and Exploration In Laboratory
关键词
旋光仪
调零
旋光方向
旋光度
比旋光度
蔗糖
polarimeter
zero set
rotatory direction
optical rotation
specific rotatory power
sucrose
geometry
作者简介
赵世民（1956-）。男，陕西洛川人，博士，教授，主要研究方向为食品化学。Tel．：0751-8120167：E—mail：shiminzha02000@yahoo．com.cn
