摘要
目的观察黄连解毒汤(HLJDT)对代谢综合征(MS)大鼠心肌的保护作用及对胰岛素信号转导的影响,探讨其保护心肌的分子机制。方法高糖、高脂及高盐饲料及20%高蔗糖水喂养(HF饮食)喂养Wistar大鼠建立MS大鼠模型,以HLJDT干预治疗12 w,检测血糖、血清胰岛素和血脂水平,Western印迹方法检测HLJDT治疗后MS大鼠心肌组织内核因子-κB(NF-κB)、磷酸化Akt(P-Akt)蛋白表达及胰岛素受体底物1(IRS-1)蛋白色氨酸磷酸化水平。结果 HLJDT治疗MS大鼠后,心肌组织内NF-κB表达及IRS-1丝氨酸磷酸化水平较MS组下降,P-AKT显著增加。结论黄连解毒汤通过抑制炎症降低IRS-1丝氨酸磷酸化水平,这可能是其保护心肌的机制之一。
Objective To investigate the effects of Huanglian Jiedu decoction （HLJDD） on myocardium and signal transduction of insulin receptor substrate in rats of metabolic syndrome （MS） , and explore its possible molecular mechanism of improving myocardial lesions. Methods MS rats were established with obese-diets （a high-fat, high-glucose and high-sah diet and sucrose solution）. MS rats in the testing group were treated with HLJDD for 12 weeks. Fasting serum glucose, insulin and blood-fat were determined, and Western blot was performed to analyze the proteins in signal transduction pathway, including nuclear factor kappa B （NF-κB） , phosphorylation Akt and tyrosine phosphorylation levels of insulin receptor substrate-1 （ IRS-1 ）. Results NF-KB protein and tyrosine phosphorylation and IRS-1 were decreased, and phospho-AKT protein was increased significantly in MS rats treated with HLJDD, compared with untreated MS rats. Conclusions HLJDD could protect myocardium from IR-mediated injury by inhibiting IRS serine phosphorylation catalyzed by inflammation in MS rats.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2012年第21期4675-4677,共3页
Chinese Journal of Gerontology
基金
山东省自然科学基金青年基金项目(ZR2010HQ059)
关键词
黄连解毒汤
代谢综合征
胰岛素受体底物1
胰岛素抵抗
Huanglian Jiedu Decoction
Metabolic syndrome
Insulin receptor substrate-1
Insulin resistance
作者简介
第一作者：李晓星（1980-），女，主治医师，主要从事心血管常见疾病的诊治。
通讯作者：季晓平（1964-），男，博士，教授，主要从事代谢综合征的机制研究。
