摘要
以苹果渣为原料,分别采用微波和纤维素酶作用提取可溶性膳食纤维,通过正交试验确定微波的适宜提取条件,结果表明:在料液比1:20,时间1.5min,微波火力为中火(功率450W),pH6的条件下,水溶性膳食纤维得率13.6%,持水力754.40%,溶胀性13mL/g。通过响应面优化试验确定纤维素酶适宜的提取条件是:纤维素酶用量0.67%,缓冲液pH5.55,酶解时间1.90h,酶解温度45℃。此时,水溶性膳食纤维提取率17.50%,持水力851.25%,溶胀性15mL/g。
The technology for extracted soluble dietary fibre from apple pomace was studied. Soluble dietary fibre was prepared by microwave and cellulase extraction. The optimal exatraction conditions of soluble dietary fibre by mi- crowave through orthogonal tests, results showed that： the ratio of solid to liquid was 1：20, 1.5 min, fire was the inter- mediate fire（450 W）, pH 6.0. Under these conditions,the yield of soluble dietary fibre reached 13.6%. The water holding capacity and swelling capacity of dietary fibre product were 754.40% and 13 mL/g, respectively. The optimal exatraction conditions of soluble dietary fibre by cellulase were established through response surface analysis（RSA）. The results showed enzyme concentration 0.67%, pH 5.55, extraction 1.90 h at 45 ℃. Under the optimized conditions, the yield of soluble dietary fibre reached 17.50%. The water holding capacity and swelling capacity of dietary fibre product were 851. 25% and 15 mL/g, respectively.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2012年第9期115-120,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
河北省科技计划项目(11221004D)
作者简介
牟建楼，女，1973年出生，硕士，讲师
通讯作者：王颉
