摘要
目的 对 5 氟尿嘧啶 (5 Fu)诱导肝癌细胞凋亡过程中caspase 3活性变化进行研究 ,以期了解caspase 3是否参与了该凋亡的调控。 方法 用 1× 10 -1、1× 10 -2 、1× 10 -3 、1× 10 -4 、1× 10 -5mol/L等不同浓度的 5 Fu处理人肝癌细胞系HepG2 ,分别作用 1、2、4、8、16h ,借助caspase 3荧光分析试剂盒检测肝癌细胞凋亡过程中caspase 3活性的变化。结果 经不同浓度的 5 Fu诱导肝癌细胞凋亡后 ,与阴性和阳性对照组比较 ,caspase 3活性均有显著增高 (P <0 .0 1) ;药物高浓度组与低浓度组之间差异亦有显著性 (P <0 .0 5 )。 5 Fu处理后 ,肝癌细胞的caspase 3活性随着作用时间的延长而迅速增高 ,至 4h后达到高峰 (733.4± 19.0 ) ,然后逐渐下降 ,但仍显著高于相应的对照组 (P <0 .0 1)。结论 caspase 3参与了 5 Fu诱导肝癌细胞凋亡的调控 ;5 Fu以时间和浓度依赖方式促进caspase 3活性增高。
Objective\ To study the change of the caspase-3 activity during the 5-fluorouracil (5-Fu) -induced apoptosis of hepatoma cells. Methods\ The selected human hepatoma cell line HepG2 was treated with 5-Fu at 1×10 -1 ,1×10 -2 ,1×10 -3 ,1×10 -4 , 1×10 -5 mol/L and 1, 2, 4, 8, 16 h respectively. The caspase-3 activity was detected by caspase-3 Fluorescent Assay Kit. Results\ After the HepG2 was treated with 10 -2 mol/L 5-Fu, the caspase-3 activity increased rapidly and reached the peak (733.4±19.0) at 4 h, then gradually fell down. But compared with the control groups, it was still significantly higher ( P <0.01). With the concentration of 5-Fu increasing, the caspase-3 activity also elevated, the group of high concentration was significantly higher then that one of low concentration ( P <0.01). Conclusions\ Caspase-3 takes part in the regulation of the 5-Fu-induced apoptosis of hepatoma cells.5-Fu promoted the increase of caspase-3 activity in a time and concentration-dependent manner.\;
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第5期401-402,共2页
Chinese Journal of Experimental Surgery
基金
湖南省科委重点资助!项目 (98SSY10 0 8)
