摘要
以高粱、紫米、大黄米为原料,以水、60%、95%乙醇为提取介质,对提取物的总酚含量、DPPH·清除能力和总抗氧化能力进行了测定。同时,分析了三种谷物60%提取物对小鼠红细胞溶血性的影响。结果表明:高粱水提物的总酚含量为2.34mg/g(GAE/DW),分别是紫米和大黄米的4倍和12.23倍;DPPH·清除率达到43.58%,而紫米和大黄米的水提物基本无清除DPPH自由基的能力。高粱、紫米、大黄米的60%乙醇提取物的DPPH·清除能力分别为89.88%、90.16%、64.70%,高粱的总抗氧化能力为0.16mmol/g(TEAC/DW),分别是紫米和大黄米的3.99倍和24.89倍。大黄米的95%乙醇提取物总酚含量较高,达到1.38mg/g(GAE/DW),具有较强的总抗氧化能力,但DPPH·清除能力较低。高粱、紫米、大黄米在2mg/mL时对H2O2诱导的红细胞氧化溶血的抑制率分别为50.77%、16.73%、22.79%。可见,高粱具有一定的保护红细胞膜的作用。
The antioxidant capacity of different ethanol extraction from three grains （sorghum, purple rice, rhubarb rice） were evaluated.During the experiment,total phenols, DPPH· scavenging ability and total antioxidant capacity were measured with the water,60% ,95% ethanol extractions of the three grains. Meanwhile, the mice blood cells hemolytic effect of 60% ethanol extracts of three grains was evaluated in vitro.The results showed that the total phenol content of sorghum water extraction was 2.34mg/g （GAE/DW）, which was 4 times the content of purple rice and 12.23 times that of rhubarb rice,respectively.DPPH · scavenging ability of sorghum water extraction was 43.58%, however, DPPH · scavenging ab In 60% ethanol concentration, the DPPH · ity for the water extraction of purple scavenging ability of three grains rice and rhubarb rice was very low. were 89.88% ,90.16% and 64.70%, respectively.The total antioxidant capacity,0.16mmol/g （TEAC/DW）, was 3.99 times and 24.89 times that of purple rice and rhubarb rice.The total phenol content of 95% ethanol extraction of rhubarb rice was 1.38mg/g （GAE/ DW） ,total antioxidant capacity was strong, but the DPPH · scavenging ability was low. The inhibition rates of hemolytic effect of three grains were 50.？？%, 16.73% and 22.79% respectively at 2mg/mL.The results showed that sorghum could protect blood cells membrane modestly.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第21期146-148,200,共4页
Science and Technology of Food Industry
作者简介
申迎宾（1981-），男，博士研究生，主要从事粮食深加工及资源利用研究。
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