摘要
目的:建立夏枯草中异迷迭香酸苷的TLC鉴别和HPLC含量测定方法,为完善夏枯草质量标准提供参考。方法:采用薄层色谱法对夏枯草及夏枯草不同部位中的异迷迭香酸苷进行定性鉴别;采用高效液相色谱法对夏枯草中异迷迭香酸苷进行含量测定,以Agilent Eclipse XDB-C18(4.6 mm×250 mm,5μm)为色谱柱,流速1.0 mL.min-1,检测波长319 nm,柱温30℃,进样体积10μL,流动相16%乙腈-84%水(含1.0%醋酸)等度洗脱。结果:异迷迭香酸苷斑点清晰,且仅存在药用部位果穗中,无干扰;异迷迭香酸苷进样在0.032 667～0.653 34μg与峰面积线性关系良好(r=0.999 9),平均回收率为98.18%,RSD 0.25%,异迷迭香酸苷在夏枯草中的含量>0.1‰。结论:所选指标为夏枯草药用部位果穗中的特征成分,所建立方法操作简单、专属性和重复性良好,可作为夏枯草的质量控制方法。
Objective： To establish an effective TLC and HPLC method for qualitative and quantitative analysis of salviaflaside in P. vulgaris which can be used to be one of the quality control of P. vulgaris. Method： Salviaflaside was identified by TLC in P. vulgaris and different parts from P. vulgaris; salviaflaside was determined by RP-HPLC. Chromatography conditions were Agilent Eclipse XDB-C18 （4.6 mm × 250 mm, 51μm） column with mobile phase of acetonitrile-1.0% acetic acid （16： 84）, UV detection wavelength was set at 319 nm and the column temperature was set at 30 ℃ with the flow rate of 1.0 mL .rain^（-1） ; the sample injection was 10 μL. Result： The TLC sports developed were fairly clear only in flower spike with no interference; good linearity of salviaflaside was obtained in the range of 0. 032 667-0. 653 34 μg （ r = 0. 999 9）. The recovery of salviaflaside was 99. 18% and RSD was 0.25%. The content of salviaflaside was more than 0. 1‰. Conclusion： Salviaflaside was characteristic compound in flower spike of P. vulgaris. The method is simple, reliable and accurate, which can be applied as the quantity control method of P. vulgaris.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第21期106-108,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
湖南省科技厅项目(2011FJ7008)
湖南省教育厅项目(11C0958)
作者简介
许招懂，硕士，高级工程师，从事新药研究及质量标准化研究，Tel：020-4051281，E-mall：xuzdl688@yahoo．com．cn
林丽美，博士，副教授，从事中药药效物质基础及质量标准化研究，Tel：0731-88458232，E-mail：lizasmile@163．com
