摘要
为了找到海桐叶器官中除草活性物质的提取分离方法,采用琼脂法研究海桐叶乙醇溶液提取物及其不同溶剂萃取相对夏至草、生菜、小麦和稗草4种受体植物幼苗生长的影响。结果表明,海桐叶乙醇溶液提取物对4种受体植物幼苗均有不同程度的抑制作用,且生菜、小麦和夏至草的敏感性明显高于稗草;进一步利用石油醚、乙酸乙酯和正丁醇对提取物进行萃取,获得含水相在内的4个萃取相,尽管它们对4种受体植物幼苗生长的抑制作用存在较大差异,但都表现出较高的活性。说明乙醇提取物及其4个不同溶剂萃取相中均存在高除草活性物质,且种类比较复杂。
In order to find the extraction method of herbicidal compounds from Pittosporum tobira（Thunb.） Ait.leaves,the effects of the ethanol extraction and different solvent extract phases of P.tobira leaves on seedlings growth of Lactuca sativa,Triticum aestivum,Lagopsis supine and Echinochloa crusgalli were studied with method of grinded plant tissue powder mixed with agar（PPA）.The results showed that,the growth of 4 kinds of receiver plants seedlings were inhibited differently by ethanolic extract of P.tobira leaves,and the susceptibility of L.sativa,T.aestivum,and L.supine were higher than that of E.crusgalli.The petroleum ether,ethyl acetate,n-butanol and water partition from ethanolic extracts had higher inhibitory effect on the growth of 4 kinds of receiver plants,although the inhibitions were different.The results suggested that,the ethanol extraction and its different solvent extract phases of P.tobira all had the high growth inhibitory substances,but the substance varieties might be more complicated.
出处
《中国农学通报》
CSCD
2012年第25期201-208,共8页
Chinese Agricultural Science Bulletin
基金
青岛市科技计划基础研究项目(11-2-4-5-(5)-jch)
山东省"泰山学者"建设工程专项经费
关键词
海桐
乙醇提取物
萃取相
受体植物
除草活性
Pittosporum tobira（Thunb.） Ait.
ethanolic extract
extract phase
receiver plant
herbicidal activity
作者简介
王霞，女，1985年出生，山东临朐人，硕士，研究方向为植物源除草活性物质。通信地址：266109青岛市城阳区长城路700号青岛农业大学化学与药学院，E—mail：taiyang_xiaoyu@163.com。
通讯作者：罗小勇，男，1963年出生，甘肃甘谷人，副教授，博士，研究方向为除草剂毒理学和植物源除草活性物质。通信地址：266109青岛市城阳区长城路700号青岛农业大学农学与植物保护学院，Tel：0532-88030472，E-mail：luo-xiaoyong@163．com。
