摘要
目的建立高效液相色谱法测定扁咽口服液中黄芩苷的含量,对其在生产过程中的转移率进行评价。方法色谱柱:Agilent Zorbax Eclipse XDB-C18(4.6 mm×150 mm,5μm);流动相:甲醇-0.5%冰醋酸溶液(45∶55);流速:1.0 ml/min;检测波长:277 nm;柱温:30℃;进样量:10μl。结果黄芩苷浓度在1.064～106.4μg/ml范围内与其峰面积线性关系良好(r=0.999 9),平均回收率(n=6)为101.26%,RSD为1.92%。黄芩苷由药材到中间品及由中间品到成品的转移率分别为17.2%～19.0%和78.7%～79.4%。结论该方法快速、简便、准确,可用于扁咽口服液的质量控制和生产工艺评价。
Objective To establish the HPLC method for determination of baicalin in Bianyan oral solution, and evaluate the transfer rate in process of production. Methods The Agilent Zorbax Eclipse XDB-Cts （4.6 mm × 150 mm, 5 μm） was used, the mobile phase consisted of methanol-0.5% acetic acid （45 ： 55） , the flow rate was 1.0 ml/min, the detection wavelength was at 277 nm, the column temperature was at 30 ℃ , the inject volume was 10 Ixl. Results A good linear correlation of baicalin was observed within the range ofl.064 - 106.4μg/ml （r=0.999 9）. The average recovery was 101.26% with RSD 1.92% （n=6）. The trans- fer rates from Radix Scutellariae to intermediate sample and to Bianyan oral solution were 17.2% - 19.0% and 78.7% - 79.4%. Conclusions The method was rapid, simple and accurate, which could be used for the quality control and processing technique evalu- ation of Bianyan oral solution.
出处
《药学实践杂志》
CAS
2012年第4期289-290,309,共3页
Journal of Pharmaceutical Practice
作者简介
[作者简介]李翔（1976-），男，主管药师．Tel：（010）66867401．E．mail：lxbj@hotmail．com．
[通讯作者]刘皈阳．Tel：（010）66867081，E-mail：liuguiy@gmail．com．
