摘要
以青天葵新鲜球茎为试材,对离体条件下青天葵的愈伤组织诱导及其植株再生进行研究。结果表明:0.1%升汞溶液处理青天葵球茎10min、玻璃培养皿有利于青天葵愈伤组织的诱导;青天葵走茎及愈伤组织在MS+1.0mg/L 6-BA培养基中培养可得到大量丛生芽及愈伤组织,多次继代培养可得到完整植株;将青天葵走茎接种于含有0.1%活性炭的MS培养基中可获得大量组培球茎。
Taking fresh corm of Nervilia fordii（Hance） Schltr.as material,the technique of callus induction and plantlet regeneration were studied.The results showed that Nervilia fordii corm treated by 0.1% HgCl 2 solution for 10min,glass culture dish were in favor of the induction and regeneration of callus.High frequency multiple shoot-bud formation from callus induced by Nerviliaf ordii corm inducing culture on a MS-medium supplemented with 1.0mg/L 6-BA.After several times of successive transfers of culture,regenerated plant could be obtained.The optimum medium for the induction of corm was a MS-medium contains 0.1%activated charcoal.
出处
《北方园艺》
CAS
北大核心
2012年第19期140-142,共3页
Northern Horticulture
基金
教育部博士点基金联合资助项目(200805720004)
关键词
青天葵
球茎
组织培养
再生植株
Nervilia fordii； corm； tissue culture； regeneration plant
作者简介
张晓丽（1987-），女，在读硕士，研究方向为中药资源的可持续利用研究和开发。E—mail：gzyxiaoli_2010@163．com
责任作者：何瑞（1972-），女，博士，研究员，研究方向为中药资源保护与可持续利用。E-mail：vipruihe@yahoo．cn．
