摘要
本试验旨在迅速、准确检测口蹄疫阴性血清和阳性血清,确立O型口蹄疫抗体金标检测试纸条的判定标准。通过3批次O型口蹄疫抗体金标检测试纸条,对180份猪、牛、羊口蹄疫阴性血清,197份猪、牛O型口蹄疫弱阳性血清,317份猪、牛、羊O型口蹄疫阳性血清,223份牛Asia 1、A型口蹄疫阳性血清,600份田间血清样品进行检测,同时用O型口蹄疫液相阻断ELISA和O型口蹄疫正向间接血凝2种方法检测相同的血清样品。对3种方法检测的结果进行分析,并且将试纸条的检测线和质控线的显色情况与O型口蹄疫液相阻断ELISA的抗体滴度相比较。确立了试纸条的诊断标准:试纸条抗体滴度1∶2-为口蹄疫抗体阴性;试纸条抗体滴度1∶8+为O型口蹄疫阳性血清。根据试纸条的显色结果与液相阻断ELISA的结果的关系,绘制了比色卡。试纸条的判定标准检测血清结果与液相阻断ELISA和正向间接血凝结果分别进行统计学分析,试纸条与液相阻断ELISA和正向间接血凝相关性分别为y=1.142x+0.7421,y=1.1845x+0.8623;相关系数(r)分别为0.9221和0.9033。结果显示,O型口蹄疫抗体金标检测试纸条的判定标准的确立和比色卡的绘制,实现半定量分析,更加迅速、准确,适于实验室、基层兽医站和养殖场使用。
The objective of the study was to establish a judging standard of colloidal gold based immunochromatographic assay strip （GICA） for detecting antibodies of serotype O of foot-and- mouth disease （FMD） rapidly. The GICA of 3 batches were tested. There were a hundred and eighty FMD negative serum samples （containing pig, cattle and sheep）, a hundred and ninety- seven FMD weak positive serum samples （containing pig, cattle）, three hundred and seventeen FMD serotype O positive serum samples （containing pig, cattle and sheep）, two hundred and twenty-three FMD serotype A and Asia 1 positive cattle sera, six hundred filed samples. To com- pare three methods of GICA, liquid-phase competition ELISA （LB-ELISA） of FMDV serotype O and Indirect Hemagglutination Test （IHA） of FMDV serotype O for detecting antibodies of FMDserotype O, all the serum samples were detected, the judging standard was established. Accord- ing to the color of test line and control line, compared with the titers of LB-ELISA, the colori metric card was drawn. The judging standard of GICA was as follows： Using a 1：2 dilution of the sample, the result was negative, and the anti-FMDV serum was negative; Using a 1 ： 8 dilution of the sample, the result was positive, which implied that the serum had serotype O FMDV positive antibodies. Compared with GICA and LB-ELISA, the colorimetric card was drawn which was helpful for reading results. The correlation of the GICA and LPBE was y = 1. 142x ＋ 0. 742 1 ; the correlation coefficient （r） was 0. 922 1; the correlation of the GICA and IHA was y = 1. 184 5x＋ 0. 862 3; the correlation coefficient （r） was 0. 903 3. These results indicated that the judging standard of GICA was prior to detecting antibodies of FMD serotype O~ the colorimetric card was easy to read, which was just monitoring the anti-FMD serotype O antibodies of immu- nized animals fast and accurately. This judging standard could promote the qualitative analysis to semi-quantitative analysis, and it had provided that it was more suitable for using in laboratory and the field.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第9期1415-1421,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
甘肃省科技厅资助
甘肃科技重大技术专项(1002NKDA037)
关键词
O型口蹄疫抗体金标检测试纸条
O型口蹄疫液相阻断ELISA
O型口蹄疫正向间接血凝
判定标准
比色卡
colloidal gold-based immunochromatographic assay strip of FMDV serotype O
liquidphase competition ELISA of FMDV serotype O
indirect hemagglutination test of FMDV serotype O
judging standard
colorimetric card
作者简介
智晓莹（1981-），女，山东龙口人，助理研究员，硕士，主要从事动物病毒学与免疫学的研究，E—mail：zhixiaoying333@163．com通讯作者：蒋韬，副研究员，Email：pcrjiang@163．com
