摘要
摘要目的：观察大鼠肾缺血再灌注损伤对肝微细结构的影响。方法：采用光镜、电镜和免疫组织化学显色对实验性大鼠肾缺血45min再灌注24h后的肝进行观察。结果：肝内出现了灶状性坏死，有两种坏死细胞出现，即肿胀式坏死和固缩式坏死。多聚ADP核糖聚合酶-1（PARP-1）阳性细胞多分布在坏死灶周边。肝组织内有中性粒细胞浸润。结论：肾缺血再灌注损伤导致肝出现灶状性细胞坏死。PARP-1介导细胞损伤构成了肝细胞坏死的一个形式。中性粒细胞可能参与了肝对肾缺血再灌注损伤的反应。
Objective： To observe microstructural changes of the liver induced by renal isehemia-reperfusion injury. Methods. Light and electron microscopy and immunohistochemical staining were used to observe hepatic microstructure after 45-min renal ischemia and 24-hour reperfusion. Results： Spotty necrosis could be seen in the liver after renal ischemia-reperfusion injury. There were two types of necrosis, congestive and pyknotic. PARP-1 mediated cell damage was involved in necrosis. In addition, leukoeytes were easily found in the liver tissues after renal isehemia-reperfusion injury. Conclusion： Spotty necrosis and PARP-1 mediated cell damage in the liver were evident induced by renaI ischemia-reperfusion iniury. Leukocytemight also be involved in the reaction of the liver to renal ischemia-reperfusion iniury.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2012年第4期441-443,共3页
Chinese Journal of Anatomy
基金
辽宁医学院归国人员启动基金（Y20101311）
作者简介
第1作者:邵姝元,E-mail:478992599@qq.com;通讯作者:包翠芬,E-mail:mianyizuhua@yahoo.com.cn
