摘要
目的:探讨加减补肺汤对肺纤维化(PF)大鼠抗氧化作用。方法:SD雄性大鼠40只,随机分为假手术组、模型组、加减补肺汤组,采用一次性气管滴注博来霉素复制PF模型(1 mg/只),假手术组滴注等量的生理盐水。术后2 d加减补肺汤组ig中药水煎液10 mL.kg-1(含生药16 g.kg-1),模型组和假手术组ig等量生理盐水,14,28 d处死动物,取血和肺脏,测定血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)和肺组织中羟脯氨酸(Hyp)的含量,实时荧光PCR测定肺组织中细胞外超氧化物歧化酶(ECSOD)mRNA的表达。结果:加减补肺汤明显改善肺纤维化大鼠的生存状态,降低肺组织中Hyp的含量,降低血清MDA含量,增加ECSOD mRNA的表达。结论:加减补肺汤能提高肺纤维化大鼠生存状态,改善纤维化指标,其机制可能与降低血清中MDA含量,上调ECSOD mRNA表达有关。
Objective：To investigate the antioxidant effects of addition and subtraction Bufei decoction（ASBD） on pulmonary fibrosis（PF） in rats.Method：Forty male Sprague-Dawley rats were divided randomly into sham operation group,model group,ASBD group.The PF model was reproduced by intratracheal injection of bleomycin.ASBD group was given traditional Chinese medicine decoction（ig,2 times to clinic dose,16 g · kg-1） starting from 2 days after operation.The sham operation group and model group were given the same amount of normal saline.The blood and lung tissue of rats were took on the 14 d and 28 d.The superoxide dismutase（SOD） activity and malondialdehyde（MDA） content in serum were measured.The content of hydroxyproline in lung tissue was detected.The mRNA of extracellular SOD（ECSOD） was measured by real-time PCR.Result：ASBD significantly improved the survival state of PF rats,reduced content of hydroxyproline in the lung tissue,decreased the level of MDA in the serum,improved the expression of ECSOD mRNA.Conclusions：ASBD could improve the survival rate of PF rats,and the fibrosis index.The mechanism might be related to decrease in the level of serum MDA and up-regulation of the expression of ECSOD mRNA.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第18期236-239,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省科技厅贵阳中医学院联合基金(黔科合中药字[2011]LKZ7026)
贵阳中医学院博士启动基金(中医研[2011]009)
贵州省中药
民族药炮制技术研究平台建设项目(黔科合社字[2009]5001)
关键词
加减补肺汤
超氧化物歧化酶
特发性纤维化
抗氧化
Objective：To investigate the antioxidant effects of addition and subtraction Bufei decoction（ASBD） on pulmonary fibrosis（PF） in rats.Method：Forty male Sprague-Dawley rats were divided randomly into sham operation group,model group,ASBD group.The PF model was reproduced by intratracheal injection of bleomycin.ASBD group was given traditional Chinese medicine decoction（ig,2 times to clinic dose,16 g · kg-1） starting from 2 days after operation.The sham operation group and model group were given the same amount of normal saline.The blood and lung tissue of rats were took on the 14 d and 28 d.The superoxide dismutase（SOD） activity and malondialdehyde（MDA） content in serum were measured.The content of hydroxyproline in lung tissue was detected.The mRNA of extracellular SOD（ECSOD） was measured by real-time PCR.Result：ASBD significantly improved the survival state of PF rats,reduced content of hydroxyproline in the lung tissue,decreased the level of MDA in the serum,improved the expression of ECSOD mRNA.Conclusions：ASBD could improve the survival rate of PF rats,and the fibrosis index.The mechanism might be related to decrease in the level of serum MDA and up-regulation of the expression of ECSOD mRNA.
作者简介
谢高宇，讲师，从事中西医结合基础研究，Tel：0851-5606145，E-mail：xiegaoyu2000@163．com
杨长福，博士，副教授，从事中医药防治肺纤维化作用机制研究，Tel：0851-5606148，E-mail：yangchangfu@126．com
