摘要
目的建立鲍氏不动杆菌(ABA)感染的快速诊断方法。方法收集临床分离的ABA48株,对其进行耐药性分析;分别用聚合酶链反应(PCR)方法、血清学方法对其快速诊断方法进行初步研究。结果 PCR对ABA的检出率为83.3%,48株菌中8株未检出;此方法的最低检出量为3.6×103 CFU;通过对60份阴性血清的检测,确定Cutoff值为0.43,对48份阳性血清进行检测,均为阳性,检出率为100.0%。结论该试验建立的PCR诊断方法和酶联免疫吸附试验(ELISA)血清学诊断方法,具有较高的特异性和敏感性,为临床ABA快速、高效诊断方法的建立提供了试验依据。
OBJECTIVE To establish the rapid diagnosis method of Acinetobacter baumannii infections.METHODS A total of 48 clinical isolates of A.baumannii were collected,the drug resistance was analyzed,a preliminary study on the rapid diagnosis methods was carried out by real-time PCR and ELISA technology.RESULTS The detection rate of A.baumannii was 83.3% by PCR,there were 8 of 48 strains of A.baumannii which were not detected;the minimum detectable amount was 3.6×103 CFU by this method;the cutoff value was identified as 0.43 through the serology test for 60 negative samples,there were 48 cases of serum which were tested positive with the detection rate of 100.0%.CONCLUSION Both PCR diagnosis method and ELISA serology diagnosis method possess high sensitivity and specificity,which provide experimental evidence for rapid and efficient diagnosis of clinical A.baumannii infections.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2012年第17期3914-3916,共3页
Chinese Journal of Nosocomiology
作者简介
通讯作者：李恪梅，E-mail：brtestlab@126.com
