摘要
为了研究干旱对液体悬浮培养发状念珠蓝细菌细胞生长及生理生化的影响,用聚乙二醇(PEG)模拟干旱,将活化后菌种分别在培养基中PEG浓度为4%、8%和12%条件下培养,以不含聚乙二醇组作为对照,测定细胞生长速率、胞外多糖和脯氨酸含量及SOD活性的变化。结果表明随着聚乙二醇浓度的增大,细胞生长速率明显下降,SOD活性呈现先升高后降低的趋势;在聚乙二醇浓度在4%和8%培养条件下,胞外多糖和脯氨酸含量随干旱胁迫的增大而增加,在聚乙二醇浓度12%培养条件下,胞外多糖和脯氨酸含量先升高后下降。模拟干旱条件下,SOD为液体悬浮发状念珠蓝细菌提供快速的响应保护,而脯氨酸和胞外多糖为细胞提供较缓慢但更为持久的保护。
Nostoc flagelliforme is a cyanobacterium with high stress resistance. This experiment is conducted in order to study the growth conditions and physiological character changes ofNostoc flagelliforme when it is cultured at different PEG concentration. Free-living cells were separately culti- vated at 4%, 8% and 12% PEG concentration in vitro for 7 days. With different drought stress there were significant differences in cell growth. With the increase of the drought stress, the activity of superoxide dismutase （SOD） declined after an obvious increase. At 4% and 8%, the contents of pro- line （Pro） and extracellular polysaccharides （EPS） increased with a rise of PEG concentration. But at 12%, the content was increased, and then de- creased. These results suggest that cell activity were apparently changed under the drought stress and SOD give N. flagelliforme a quick protection while the EPS, Pro give it a permanent one.
出处
《中国酿造》
CAS
2012年第8期82-84,共3页
China Brewing
基金
国家自然科学基金(20776112)
国家自然科学基金(20376061)
关键词
发状念珠蓝细菌
聚乙二醇
干旱胁迫
液体悬浮培养
生理生化
Nostoc flagellifonne
polyethylene glycol（PEG）
drought stress
liquid suspension culture
physiology and biochemistry
作者简介
丁振，男，山东人，博士研究生，研究方向为发酵工程；
贾士儒，教授，通讯作者。
