摘要
采用溶液培养法,研究了镉胁迫对绿豆和箭舌豌豆幼苗根基因组DNA多态性和活性氧产生的影响。结果表明:Cd胁迫降低了2种植物根尖基因组DNA模板的稳定性,导致DNA多态性发生了变化,包括RAPD谱带的增加、缺失及其荧光强度的改变;同时,Cd胁迫抑制了根的伸长生长,促进了根内超氧阴离子(O2.-)产生和过氧化氢(H2O2)积累;Cd胁迫下2种植物根的伸长与其根内O2.-产生、H2O2积累以及DNA多态性呈负相关,DNA多态性与Cd胁迫下H2O2积累呈正相关。
The effects of cadmium （Cd） on the reactive oxygen productions and genome DNA polymorphism changes in roots of Phaseolus aureus Roxb. cv. VC3762 （Cdsensitive species） and Vicia sativa L. cv. 333/A （Cdresistant species） seedlings were investigated using hydroponic solution culture. The results showed that Cd stress decreased the genomic template stability and induced the changes of the genome DNA polymorphism in the root tips of the two species, manifesting the variation of band intensity, loss of normal bands, and appearance of new bands. Meanwhile, Cd stress inhibited the root growth and increased the production of O2-· and the accumulation of H2O2 in the roots. The root length of the two species under Cd stress was negatively correlated with the production of O2-·, accumulation of H2O2, and genome DNA polymorphism in root tip tissues, and the genome DNA polymorphism was positively correlated with the accumulation of H2O2 under Cd stress.
出处
《生态学杂志》
CAS
CSCD
北大核心
2012年第9期2330-2336,共7页
Chinese Journal of Ecology
基金
国家自然科学基金项目(30571107和31160053)资助
关键词
CD胁迫
豆科植物
活性氧
DNA多态性
Cd stress
leguminous species
reactive oxygen
DNA polymorphism.
作者简介
张芬琴，女，1963年生，博士，教授，主要从事植物抗性生理生化和环境保护方面的教学与科研工作。E—mail：fenqinzh@hxu．edu．cn
通讯作者E-mail：zgshen@nj．du．edu．cn
