摘要
采用RT-PCR和RACE(cDNA末端快速扩增)方法克隆黄颡鱼MyoD(myogenic differentiation antigen,成肌分化抗原)基因的cDNA全长序列,对其进行生物信息学和在雌雄个体组织中的表达差异分析.结果表明,黄颡鱼MyoD基因编码区由810个碱基组成,编码269个氨基酸,其中第1～89个氨基酸为Basic区,第90～141个氨基酸为bHLH结构;黄颡鱼的MyoD基因与斑点叉尾鮰的同源性最高为93%.在雌雄成体组织中的表达分析显示,雄性个体中肌肉和胃组织中的表达量极显著高于雌性个体(p<0.01),雌性个体的肾脏和鳃组织中的表达量显著高于雄性(p<0.05).通过MyoD基因在雌雄个体中表现出的组织表达差异,认为这可能是造成黄颡鱼雌雄生长差异的重要因素之一.
MyoD gene was obtained using reverse transcription-polymerase chain reaction （RT-PCR） and rapid- amplification of cDNA end （RACE） method from yellow catfish （Pelteobagrus fulvidraco）. The bioinforrnatics of the gene and differential expression in female and male adult tissues were analyzed. The results show that MyoD ORF consists of 810 nucleotides encoding 269 amino acids, including a bHLH domain which is composed from 90th to 141th. The MyoD shows high homogeneity to that of channel catfish （93%） and other animals, suggesting MyoD is highly conserved through evolution. The real-time PCR analysis reveals that the expression level in muscle and stom- ach of male are higher than that of female＇s significantly （p〈0.01）, while the level in kidney and gill of female are higher than that of male＇s significantly （p〈0.05）. The expression difference which exists between female and male is probably one of important factors relating to the growth dissimilarity.
出处
《武汉大学学报（理学版）》
CAS
CSCD
北大核心
2012年第4期347-353,共7页
Journal of Wuhan University:Natural Science Edition
基金
"十二五"农村领域国家科技计划课题(2011AA100401)
中央级公益性科研院所基本科研业务费专项(2011JBFZ01)资金项目
关键词
黄颡鱼
成肌分化抗原
生物信息学
表达
Pelteobagrus fulvidraco
myogenic differentiation antigen
bioinformatics
expression
作者简介
梁宏伟，男，助理研究员，博士生，现从事水产遗传育种研究．E-mail：lianghw@yfi．ac.cn
