摘要
探索有机磷酸酯类化合物对大鼠γ 氨基丁酸受体的体外影响 ,为有机磷酸酯类化合物急性中毒的体内研究提供理论依据。用大鼠脑神经末梢微囊GABA受体为模型 ,观察二嗪农、梭曼、益棉磷、乙硫磷、杀螟松、丙氟磷等有机磷酸酯类化合物对大鼠GABA受体的体外反应变化。结果观察到10mmol/L益棉磷、杀螟松、丙氟磷或 5mmol/L二嗪农、梭曼均明显降低大鼠GABA受体与3 H GABA的结合率 (P <0 0 1) ,0 1mmol/L梭曼或 0 5mmol/L二嗪农仍明显降低GABA受体与3 H GABA的结合率 (P <0 0 5 )。表明有机磷酸酯类化合物在一定浓度下体外反应能显著降低大鼠脑GABA受体与3 H GABA的结合率 ,GABA受体参与了某些有机磷酸酯类化合物急性中毒过程。
To study the mechanism of GABA receptor of organophosphates(OP)in vitro in order to give the evidencesof studying Op in vivo.Experiment use the model of rat's microsac to observe the change of 3H GABA bining to GABA receptor with diazino,soman,DFP, fenitrothion,azinphos ethyl,ethion acting in vitro.The 3H GABA binding of rat's microsac was significant decreased when 10mmol/L diisopropylfluorophosphate(DFP),fenitrothion,azinphos ethyl or 5mmol/L diazinon,soman were acted on the rat's microsac in virto(P<0\^01).0\^1mmol/L soman or 0\^5mmol/L diaznon were acted on the rat's microsac in vitro,the 3H GABA binding were still significant decreased (P<0 05).The binding of GABA receptor was decreased when organophosphates were acted on the rat's microsac in vitro.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第7期599-600,共2页
Chinese Journal of Public Health
