摘要
目的观察大鼠急性心肌梗死时心肌中细胞间黏附分子-1（ICAM-1）和白细胞介素（IL）-6的表达及银杏叶提取物对上述指标的影响，探讨银杏叶提取物对大鼠急性心肌梗死损伤心肌的保护作用。方法选择健康雌性sD大鼠109只，结扎左冠状动脉前降的方法制备急性心肌梗死模型，随机分为心肌梗死模型组（A组，n=38）、银杏叶治疗组（B组，H=39）和假手术组（C组，I=32）。A组-M＊t：L左冠状动脉前降支；B组术前30rain尾静脉注射银杏叶提取物2m∥kg；C组仅在前降支留置一松结，不结扎。各组再按术后观察时点（2h、6h、48h,7d）随机分为4个亚组。分别于各观察时点采集各亚组大鼠心脏标本。苏木素-伊红（HE）染色光镜下观察形态学变化，免疫组织化学法检测ICAM-1和IL-6的表达。结果（1）HE染色显示A组大鼠左心室前壁心外膜下心肌细胞数量减少，心肌问质充血、水肿、心肌纤维溶解、片状坏死，伴有多量炎性细胞浸润，坏死心肌范围超过心壁厚度的1／2以上，心内膜侧仍见大量存活心肌细胞；B组心肌损伤程度较A组减轻；C组大鼠左心室前壁心外膜下散在心肌间质充血、水肿，未见心肌细胞坏死。（2）免疫组织化学结果显示，在术后2h、6h、48h、7d各时间点A组ICAM-1（493．02±86．32、550．05±87．36、695．05．4-117．35、807．56±132．05）干口IL-6（153．02±26．32、179．05±28．36、185．35±37．05、161．18±31．05）、B组ICAM一1（434．83±，75．63、508．45±96．21、596．284-105．36、679．25±129．54）和IL-6蛋白含量（137．83±33．63、155．42±26．21、157．28±25．36、135．25±26．54）均高于C组的ICAM-1（310．59±82．31、330．27±80．32、320．95±81．36、341．84±82．35）和IL-6（87．59±22．31、97．27±26．32、93．95±19．36、91．84±24．35），差异有统计学意义（P〈0．05）；A组各观察时点1CAM-1和IL-6蛋白含量均高于B组，差异有统计学意义（P〈0．05）。结论（1）大鼠急性心肌梗死模型心肌中ICAM-1和IL-6的表达上调，上述指标可反映心肌损伤的程度和冠脉病变范围。（2）大鼠急性心肌梗死中应用银杏叶提取物干预，可下调心肌中ICAM-1和IL-6的表达，减轻大鼠心肌损伤程度，证实银杏叶提取物有较好的心肌保护作崩。
Objective To study the expression of intercellular adhesion molecule-1 （ ICAM-1 ） and interleukin （IL） -6 in myocardium with acute myocardial infarction in rats and the influence of ginkgo biloba extract on them, and to explore the mechanism of protective effects of ginkgo biloba extract on acute infarcted myocardium. Methods One hundred and nine healthy female SD rats were selected to make acute myocardi- al infarction model by ligation of the left anterior descending （LAD） coronary artery, and then they were ran- domly divided into model group （group A, n = 38 ）, ginkgo biloba treatment group （group B, n = 39 ）, and sham operation group （ group C, n = 32）. In group A, the left anterior descending （LAD） coronary artery. was ligated solely; in group B, Ginkgo biloba extract （2 mg/kg body weight） was intravenously injected 30 rain before ligation of LAD coronary artery; in group C, a loose knot was made around the first descending branch of LAD coronary artery, without ligation. Each group was then randomly divided into 4 subgroups,which were observed 2 h, 6 h, 48 h, and 7 days after operation, respectively. Blood and heart samples were collected at every time point. Morphological changes were observed by HE staining method under the light microscopy, and ICAM-1 and IL-6 expression was detected by using immunohistochemistry. Results By u- sing HE staining, in group A, the number of myocardial cells was decreased, and myocardial interstitial con- gestion, edema, myocardial fiber dissolution, flaky necrosis and infiltration of inflammatory cells were ob- served. Range of myocardial ilffaretion was more than 1/2 of cardiac wall, and large number of viable myo- cardial cells still could be seen in the other side of cardiac wall; In group B, myocardial injuries were signifi- cantly milder than those in group A; In group C, there were myocardial interstitial scattered congestion, ede- ma in subepicardium of left ventricular anterior wall, but without myocardial necrosis. Immunohistochemistry revealed that at 2nd h, 6th h, 48th h, and 7th day, the protein levels of ICAM-1 （493.02 ± 86. 32,550. 05 ±87.36, 695.05± 117.35, 807.56±132.05） and IL-6 （153.02±26.32, 179.05 ±28.36, 185.35± 37.05, 161.18 ±31.05） in group A, and those [ （ ICAM-1 ： 434. 83 ± 75.63, 508.45 ± 96. 21, 596. 28 ± 105.36, 679. 25± 129. 54 ） and （ IL-6 ： 137. 83 ±33.63, 155.42 ± 26. 21, 157.28±25. 36, 135.25 ±26. 54）] in group B were significantly higher than those in group C （ICAM-1 ： 310. 59 ± 82. 31, 330. 27± 80. 32, 320. 95 ± 81.36, 341.84 ± 82. 35 ; IL-6 ： 87.59 ± 22. 31, 97.27 ± 26. 32, 93.95± 19. 36, 91.84± 24. 35）, respectively （P 〈0. 05）. ICAM-1 and IL-6 protein levels in group A were significantly higher than those in group B at each time point, respectively （P 〈 0. 05 ）. Condusion （ 1 ） The expression of ICAM-1 and IL-6 was upregnlated in acute infraeted myoeardium; （2） The application of ginkgo biloba extract can downregnlate the expression of ICAM-1 and IL-6, and reduce the extent of myocardial injury, suggesting the protective effect of gnkgo biloba extract on isehemie myocardium.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第8期1443-1446,共4页
Chinese Journal of Experimental Surgery
基金
中国博士后基金资助项目（57068）
河南省科技创新人才基金资助项目
关键词
心肌梗死
银杏叶提取物
心肌保护
黏附分子
白细胞介素-6
Myocardial infarction
Ginkgo biloba extract
Myocardial protection
Adhesion molecule
Interleukin-6
作者简介
通信作者：付庆林，Email：fuqinglin@xxmu．edu．cn
