摘要
采用FIASCO方法,从糙皮侧耳(Pleurotus ostreatus)菌株ACCC 50838分离简单重复序列分子标记(Simple sequence repeat,SSR),所得标记用于分析我国52个侧耳(平菇)栽培菌株多样性,对于SSR标记相同的菌株再进行ISSR PCR分析。结果表明:18个SSR标记在供试菌株中的等位基因的数量为3～12个,多态性指数(PIC)为0.34～0.84;除了2组(ACCC50618,ACCC 50866和ACCC 50915;ACCC 50249和ACCC50476)共5个菌株之外,其他47个菌株都能利用这18个SSR标记进行有效的鉴别。SSR标记相同的的菌株,其ISSR、体细胞不亲合性和出菇性状(温型、菌盖颜色)也相同,这些菌株可能是同物异名。利用这18个SSR标记分析52个菌株的遗传多样性,相似性指数为0.755～0.9995。根据相似性指数进行聚类分析,52个菌株分成两个大的类群,分别为糙皮侧耳(P.ostreatus)和肺形侧耳(P.pulmonarius)。结合这些栽培菌株的农艺性状和SSR分子标记的聚类分析结果,我国平菇栽培菌株的遗传多样性非常丰富。研究表明SSR分子标记技术在平菇遗传多样性和菌株鉴别中具有广泛的应用前景。
Oyster mushrooms represent one of the most important groups of commercially cultivated edible mushrooms worldwide.In this study,18 novel polymorphic simple sequence repeat（SSR） markers were developed and characterized from Pleurotus ostreatus,strain ACCC 50838.The number of alleles detected for each SSR locus varied between three and twelve across the 52 oyster mushroom cultivars,48 of which were previously designated as P.ostreatus and four as P.pulmonarius.The polymorphism information content（PIC） of the SSRs ranged from 0.34 to 0.84.All 52 cultivars were differentiated using the 18 SSR markers except for two small groups,comprised of three and two strains respectively,which were also indistinguishable on the basis of ISSR profiles,somatic compatibility patterns and agronomic traits such as temperature type and pileus color.Similarity matching（SM） coefcients for all the SSR bands ranged between 0.755 to 0.9995,and a dendrogram based on these values separated the 52 cultivars into two distinct groups（P.ostreatus and P.pulmonarius） at the similarity level of 0.79.A high degree of heterogeneity among the Chinese cultivars was revealed by the SSR markers,which have considerable potential for evaluating genetic diversity essential to future oyster mushroom breeding programs.
出处
《食用菌学报》
北大核心
2012年第2期15-25,共11页
Acta Edulis Fungi
基金
Sponsored by the Foundation of the National Natural Science Foundation of China(No.31101588)
关键词
简单重复序列
平菇类
糙皮侧耳
肺形侧耳
遗传多样性
鉴别
Simple sequence repeat（SSR）
oyster mushroom
Pleurotus ostreatus
Pleurotus pulmonarius
genetic diversity
strain differentiation
作者简介
Corresponding author:张瑞颖.Tel:+86-611-82108683,E-mail:caufarmer@163.com.
