摘要
目的:探讨人巨细胞病毒(HCMV)在动脉粥样硬化(AS)发生和发展中的作用及可能的机制。方法和结果:采用组织培养技术和流式细胞计数仪观察HCMV对离体培养的HUVEC凋亡的影响。实验发现无血清培养和TNF诱导的细胞凋亡率较正常对照组升高,而CMV感染组凋亡率明显降低。用流式细胞仪检测发现,在DNA直方图上,在G1峰左侧可呈现亚二倍体核峰型(亚G1峰,即凋亡峰)。而正常的细胞经流式细胞仪检测,没有亚二倍体细胞群的峰型;CMV可以抑制HUVEC的一氧化氮(NO)的合成,并且呈浓度依赖性。结论:HCMV感染HUVEC后能够抑制HUVEC的细胞凋亡,可能的介导因素为被HCMV感染的HUVEC其NO合成减少。
Objective:To study the role of human cytomegalovirus(HCMV)in the initiation and progression of atherosclerosis(AS).Methods and Results:The effect of HCMV on the apoptosis of cultivated human umbilical vein endothelial cell(HUVEC)was assayed by flow cytometry.The HUVEC apoptosis rate induced by trmour necrosis factor(TNF)and cell culture without serum was reduced by HCMV infection.HCMV could inhibit with concentration-dependence.Conclusion:HCMV infection could inhibit apoptosis of cultured human umbilical vein endothelial cell(HUVEC),the possible mechanism is due to the reduced synthesize of nitrous monoxide(NO)in HUVEC.
作者
王德胜
黄从新
杨波
尹丽娅
王晶
李晓艳
Wang Desheng;Huang Congxin;Yang Bo(Department of Cardiology,First Affiliated Hospital,Hubei Medical University,Wuhan 430060,China)
基金
湖北省科学基金资助项目,编号93J74
关键词
巨细胞病毒
HCMV
细胞凋亡
动脉粥样硬化
cytomegaloviruses
umbilical veins
endothelium,vascular
apoptosis
nitrous monoxide
