摘要
目的对广东省深圳市2010年首例临床诊断为手足口病死亡患儿的粪便和咽拭子标本进行病原体型别鉴定,从而追踪其病原的可能来源。方法先用实时荧光定量反转录-聚合酶链反应(rRT-PCR)方法检测病毒核酸,再用细胞培养方法从粪便和咽拭子标本中分离病毒,并将分离到的毒株提取核酸后,用普通RT-PCR方法分别扩增肠道病毒71型(EV71)和柯萨奇病毒A组16型(Cox A16)的VP1区全长基因并进行序列测定,序列测定结果与不同国家和地区的肠道病毒株A、B、C基因型代表株进行同源性比较和进化树分析。结果该病例的2份标本均为EV71核酸阳性,Cox A16核酸阴性;毒株基因经过核苷酸和氨基酸同源性分析,确定该病例病原体为EV71 C4亚型。结论 2010年深圳市首例手足口病死亡病例的病原体为EV71的C4亚型。
Objective To identify the pathogen of the first clinical death case of hand, foot and mouth disease （HFMD） in Sbenzhen in 2010 and find the infection source. Methods Fluorescent RT-PCR was conducted to detect viral nucleic acid of the virus, and virus strains were isolated from stool and throat swabs of the case, then conventional RT-PCR was conducted to amplify full length genes of EV71 and Cox A16 in VPI region of nucleic acid extracted from isolated strains. The gene sequencing, comparison of homology with the representative strains of enteroviruses （ genotype A, B, C ） isolated in different countries and phylogenetic tree analysis were carried out. Results The two swabs of cases were all positive for EV71 but negative for Cox A16. The gene sequence analysis indicated that the virus isolated belonged to C4 subtype of EV71. Conclusion The pathogen of the first death case of HFMD in Shenzhen was EV71 C4.
出处
《疾病监测》
CAS
2012年第7期512-515,共4页
Disease Surveillance
基金
广东省医学科学技术研究基金(No.A2011554)~~
关键词
手足口病
肠道病毒
病原学
序列分析
系统进化树
hand, foot and mouth disease
enterovirus
etiology
sequence analysis
phylogenetic tree
作者简介
杨洪，女，广东省普宁市人，主任技师，主要从事肠道病毒的研究及检测工作通信作者：杨洪，Tel：0755—25637131，Email：szgangqiyang@163．com
