摘要
目的探讨灵芝三萜对急性单核细胞白血病细胞株THP-1作用及其可能的机制。方法采用不同水平灵芝三萜作用于THP-1细胞48小时后,噻唑兰(MTT)比色法测定细胞增殖抑制率;流式细胞术(FCM)检测细胞周期分布;钙依赖磷脂结合蛋白异硫氰酸荧光素/碘化丙啶(annexin V-FITC/PI)双染法流式细胞仪检测凋亡率的变化。结果 MTT测定细胞增殖抑制率,与灵芝三萜0mg/L比较,灵芝三萜80、120、160、200mg/L抑制THP-1细胞增殖,抑制率逐渐升高(P<0.01),分别为(21.58±3.15)%、(54.36±4.49)%、(62.10±4.23)%和(70.20±4.21)%,呈剂量依赖性。Annexin V-FITC/PI双染显示,80、120、160、200mg/L的灵芝三萜作用THP-1细胞48小时后产生诱导凋亡作用,早期凋亡率分别为(18.6±4.30)%、(28.1±5.88)%、(40.46±6.39)%、(54.22±2.00)%,随着灵芝三萜浓度的增加有增加趋势(P<0.01)。FCM检测细胞周期,80、120、160、200mg/L的灵芝三萜作用THP-1细胞48小时后,G2/M期、S期细胞数明显减少,G0/G1期细胞数明显增加,与对照组比较差异有统计学意义(P<0.01)。结论灵芝三萜有抑制THP-1细胞增殖的作用,其可能的机制是阻止细胞周期在抑制G1期和诱导细胞凋亡。
Objective To explore the effect of ganoderma triterpenoids on acute monocytic leukemia cell line THP-1 and＇its possible mechanism. Methods After being treated with ganoderma triterpenoids at different dosages, cell proliferation rate was measured by methylthiazol tetrazolium colorimetry methyl thiazolyl tetrazolium （MTT） assay;distribution of cell cycle was examined by flow cytometry（FCM） ;early apoptosis rate was measured by flow cytometry through annexin V-FITC/PI apoptosis detection kit （annexin V-FITC/PI） double staining. Results Inhibition of cell proliferation was determined with MTT, compared with ganoderma triterpenes 0 mg/L,THP-1 cell proliferation inhibition rate with ganoderma triterpenes in 80,120,160,200 mg/L were respectively（21.58± 3.15）%, （54.36 ± 4.49）%, （ 62.10 ± 4.23） % and （ 70.20 ± 4.21 ） % in a dose dependent manner （ P 〈 0.01 ）. By FCM cell cycle, compared with that of control group, ganoderma triterpenes in 80,120,160,200 mg/L at 48 h had effect on THP-1 cells and reduced the number of cells in G2/M phase and S phase while increased the number of cells in G0/G1 phase significantly（ P 〈0.01）. Ganoderma triterpene affected THP-1 cells after the induction of apoptosis, the early apoptosis rates were （18.6 ± 4.30）%, （28.1 ± 5.88）%, （40.46 ± 6.39）%, （54.22 ± 2.00）%, with the increase in the concentration of ganoderma triterpene in 80,120,160,200 ug/L,there was an increasing trend （ P 〈0.01）. Conclusion Ganoderma triterpenoids can inhibit the proliferation of THP-1 cells and the possible mechanism is to prevent the cell cycle in G1 phase and induction of apoptosis.
出处
《临床荟萃》
CAS
2012年第15期1325-1328,共4页
Clinical Focus
作者简介
第一作者赵哲为武汉大学人民医院生物医学工程硕士专业在读研究生
通信作者：高清平，Email：gao．qingping@163．com
