摘要
目的研究玉足海参(Holothuria leucospilota)具有生物活性的三萜皂苷类成分。方法应用大孔树脂柱色谱、正相硅胶柱色谱、反相硅胶柱色谱和HPLC对玉足海参体内三萜皂苷活性成分进行分离纯化,根据化合物的理化性质和波谱数据鉴定其结构。结果分离并鉴定了3个三萜皂苷类化合物,分别为:leucospilotaside D(1)、holothurin B(2)和holothurin B2(3)。化合物1～3对人宫颈癌细胞HeLa,人胃癌细胞BEL-7402和人乳腺癌细胞MCF-7的抑制率较强。结论化合物1为首次从玉足海参中分离得到。3个化合物均显示显著的体外细胞毒活性。
OBJECTIVE To study the bioactive triterpene glycosides in sea cucumber Holothuria leucospilota. METHODS Triterpene glycosides in the sea cucumber were isolated and purified by column chromatography on DA-101, silica gel, reversed-phase silica and reversed HPLC. Their chemical structures were identified on the basis of chemical properties and spectral data~ The eytotox- ieities of the compounds obstained were tested with human cervical carcinoma HeLa cells, gastric carcinoma BEL-7402 cells, and breast carcinoma MCF-7 cells. RESULTS Three triterpene glycosides were obstained from the sea cucumber studied and their struc- tures were identified as leucospilotaside D （ 1 ）, holothurin B （2）, and holothurin B2 （3）. Their inhibition ratios for HeLa, BEL-7402, and MCF-7 cells were high. CONCLUSION Compound 1 is obstained from Holothuria leucospilota for the first time. All glycosides show marked in vitro antitumor activity.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第15期1194-1198,共5页
Chinese Pharmaceutical Journal
基金
国家高技术研究发展计划"863计划"(2006AA09Z417)
国家自然科学基金资助项目(20772155)
作者简介
韩华，博士，女，讲师研究方向：海洋生物资源利用，海洋生物活性物质研究
通讯作者：李玲，女，教授研究方向：海洋天然活性物质Tel：13901978639E-mail：lilingty@126．com
