摘要
目的采用HPLC测定片剂中阿戈美拉汀的含量。方法色谱柱为PhenomenexLuna-C18（250mmx4．6mm，5μm），流动相为甲醇-0．05mol·L-磷酸二氢钠溶液（40：60），流速为1．0mL·min-1，检测波长为230nm，柱温为25℃。结果线性回归方程为Y=I．979×100，-1．258×10^4（r=0．9999，n=5），线性范围为1．07-10．7μg·mL^-1，平均回收率为98．73％（n=9），RSD为1．28％。结论所建方法简便、准确、专属，可用于片剂中阿戈美拉汀的含量测定。
OBJECTIVE To establish a method for the content determination of Agomelatine tablets by HPLC. METHODS The HPLC method was performed on Phenomenex Luna-C18 column （250 mm×4.6mm, 5μm） with mobile phase of methanol-0.05 mol.L-1 sodium dihydrogen phosphate （40 ： 60）. Flow rate was 1.0 mL.min-1. Detective wavelength was set at 230 nm and the column temperature was set at 25℃. RESULTS The calibration curve of Agomelatine was linear Y=1.979 ×105X-1.258×104 （r=0.999 9, n=5） in the range of 1.07-10.7μg.mL-1. The average recovery of Agomelatine was 98.73% （n=9） with RSD of 1.28%. CONCLUSION The method is simple, accurate, specific and it can be used for the determination of the content of Agomelatine tablets.
出处
《中国现代应用药学》
CAS
CSCD
2012年第7期652-655,共4页
Chinese Journal of Modern Applied Pharmacy
作者简介
王毓菁，女，硕士生Tel：13815894302E-mail：lollipopwyj@126．com
通讯作者：张根元，男，副研究员Tel：13951791063E-mail：zhanggenyuan63@126．com
