摘要
以单分散交联聚甲基丙烯酸环氧丙酯(PGMA/EDMA)微球作为基质载体,溶菌酶为模板分子,β-环糊精和丙烯酰胺为复合单体,N,N′-亚甲基双丙烯酰胺为交联剂,采用表面印迹法制备了溶菌酶分子印迹聚合物;以此聚合物为固定相进行前沿色谱分析和识别性能研究.结果表明,该分子印迹聚合物对模板分子溶菌酶具有特异识别能力,基于此可以分别实现溶菌酶与牛血清白蛋白、卵白蛋白及肌红蛋白的色谱分离.
Lysozyme-based molecularly imprinted polymers （Lys-MIPs） were prepared with POMA/EDMA beads as a carrier material, lysozyme as the template molecule, β-cyclodextrin and acrylamide as the double functional monomers, N,N＇-methylene bisacrylamide as the crosslinker. The prepared Lys-MIPs were used as a new HPLC packing material, based on which the related study on its recognition was conducted through the frontier chromatography. The results showed that Lys-MIPs had specific recognition for lysozyme, which helped achieve the separation between lysozyme and three individual competitive proteins, including bovine serum albumin （BSA）, ovalbumin（OB） and myoglobin （Myo）.
出处
《陕西师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第4期46-50,共5页
Journal of Shaanxi Normal University：Natural Science Edition
基金
国家自然科学基金资助项目(81173025)
关键词
聚甲基丙烯酸环氧丙酯(PGMA/EDMA)微球
溶菌酶
分子印迹
色谱分离
poly （glycidylmethacrylate-co-ethylenedimethacrylate） （PGMA/EDMA） beads
lysozyme
molecular imprinting
chromatographic separation
作者简介
韩艳婷，女，硕士，主要研究方向为色谱分析．E-mail：hyt041142005@126．com．
通信作者：张静，女，副教授，博士．E-mail：zhanNing8902@snnu．edu．cn．
