摘要
采用细胞破碎、硫酸铵分级沉淀和Sephadex G-75凝胶过滤的方法,对黑曲霉(Aspergillus niger)J6产生的有机磷农药降解酶进行分离和纯化,并对其酶学性质进行研究。结果显示:黑曲霉J6有机磷农药降解酶定位在细胞膜上,其分子量为66 000。该降解酶最适pH值为7.0,最适反应温度为70℃,Fe3+、Cu2+、Li+、Zn2+、Mg2+、NaN3、EDTA、SDS、PSMF对该降解酶有不同程度的激活作用,而Mn2+对其有轻微的抑制作用,Ca2+和Triton对其有强烈的抑制作用。
A organophosphorus hydrolase from strain Aspergillus niger J6 was isolated and purified by means of cell disruption, ammonium sulfate precipitation and Sephadex G-75 chromatography, and its enzymatic properties were studied. The results showed that the degradation enzyme was located on the cell membrane, with its molecular weight being 66 000 by SDS-PAGE. The optimal reactive temperature and pH of the degradation enzyme were 70 ℃ and pH 7.0. Some metal i- ons（ Fe3＋, Cu2＋, Li＋, Zn2＋, Mg2＋） and chemical reagents（ NAN3, EDTA, SDS and PSMF） showed activation on the deg- radation enzyme, while Mn＋ displayed slight inhibition, and Ca2＋ Triton had strong inhibition.
出处
《江苏农业学报》
CSCD
北大核心
2012年第3期549-554,共6页
Jiangsu Journal of Agricultural Sciences
基金
2009年河南省重大公益性科研招标项目(091100910500)
关键词
黑曲霉
有机磷农药降解酶
酶学性质
Aspergillus niger
organophosphorus pesticide-degrading enzyme
enzymatic property
作者简介
作者简介：王继雯（1972-），女，河南驻马店人，硕士，助理研究员，主要从事农业微生物技术研究。（E—mail）wangjiwen2005@126．com.
通讯作者：谢宝恩，（E—mail）xbaoen@163．com
