摘要
以亚洲百合‘普瑞头’(Lilium asitic hybrids cv.Prato)鳞片为外植体,以MS为基本培养基,通过增加不同激素种类和浓度进行了组织培养快速繁殖技术的研究。结果表明,诱导不定芽的最适培养基为MS+0.5mg.L-1 6-BA+0.1mg.L-1 NAA,诱导率最高达85.0%,平均每块分化的芽数最多(3.6个);再生小鳞茎鳞片诱导不定芽的最适培养基为MS+1.0mg.L-1 6-BA;生根的最适宜培养基为1/2MS+0.5mg.L-1 IBA,生根率为86.7%;移栽后生长良好,成活率达到85.0%。
The rapid propagation technique was studied in Lilium asitic hybrids cv. Prato with the bulb scales and leaves in vitro as explants and MS as basic medium with different hormones and concentrations. The results showed that the best medium for adventitious bud induction from bulh scales and leaves was MS＋0.5 mg·g^-1 6-BA＋0.1 mg·L^-1 NAA, with which the highest induction rate was 85.0%, and the average number of shoots from every explant was 3. 6. The best medium for adventitious buds induced from bulb scales was MS＋1. 0 mg·L^-1 6-BA. The optimum medium for taking root was 1/2 MS＋0.5 mg·L^-1 IBA,and the rooting rate was 86.7%. The transferred plants, with a survival rate of 85.0%, grew well.
出处
《草业科学》
CAS
CSCD
北大核心
2012年第7期1077-1083,共7页
Pratacultural Science
基金
中央高校基本科研业务费专项资金项目(DL12CA17)
关键词
'普瑞头’
鳞片
组织培养
快繁
Lilium asitic hybrids cv. Prato
bulb scales
tissue culture
rapid propagation
作者简介
张彦妮（1974-），女，山西大同人，博士，主要研究方向为园林花卉的繁育栽培及育种。E—mail：zhangyanni808@126．com
