摘要
用添加氧载体(油酸、豆油)、表面活性剂(Triton-X100)及H2O2的方法,改善L-苯丙氨酸发酵体系中的氧传递速率,以提高苯丙氨酸的产量。实验结果表明,在发酵0h添加1%的豆油、3%的油酸均可使产酸提高,分别可以使L-phe产量提高21.1%和39.5%;发酵0h同时加入3%油酸和0.05%Triton-X100时,提高产量78.95%;发酵12h添加0.075%H2O2,可以提高产苯丙氨酸产量18.42%。
Oxygen vectors (oleic acid, soybean oil), surfactant (Triton-X100) and H2O2 can effectively improve the oxygen transfer rate in the fermentation system, and increase the yield of phenylalanine. Experimental results showed that, adding 1% soybean oil and 3 % oleic acid before fermentation could make the L-phe production increased by 21.1% and 39.5% respectively; while adding 3% oleic acid and 0.05% Triton-X100 at the same time could make L-phe production increased by 78.95% ; adding 0. 075% H2O2 at 12 h fermentation could make L-phenylalanine production increased by 18.4%.
出处
《氨基酸和生物资源》
CAS
2012年第2期39-42,共4页
Amino Acids & Biotic Resources
基金
国家发改委"2010年国家技术产业化微生物制造专项"
作者简介
翁雪清(1982-),男,工程师,现主要从事发酵工艺控制方面的工作.Email:646371220@qq.com
通信联系人 E-mail:wsg5014242@yahoo.com.cn