摘要
目的:对照盐酸氟桂利嗪的效应,探讨电针调节椎-基底动脉供血不足(VBI)家兔耳蜗核传出性神经递质γ-氨基丁酸B受体(GABABR)表达对耳蜗Corti器形态和功能的影响。方法:以组织硬化剂-775注射液注射致家兔左侧颈椎横突软组织建立椎动脉型颈椎病VBI模型,对照组注射生理盐水。注射后第6周开始,电针组进行"凤池""听宫""外关"穴电针刺激,氟桂利嗪组给予盐酸氟桂利嗪胃肠灌注。结果:干预2周后,模型组、电针组和氟桂利嗪组听神经复合动作电位(CAP)阈值显著高于对照组(P<0.001);电针组和氟桂利嗪组显著低于模型组(P<0.001),模型组、电针组和氟桂利嗪组高频短纯音CAP阈值高于低频短纯音阈值(P<0.05)。模型组GABABR阳性产物积分光密度显著低于对照组(P<0.001),电针组和氟桂利嗪组显著高于模型组(P<0.01),与对照组无显著差异(P>0.05)。模型组、电针组和氟桂利嗪组残存毛细胞计数显著低于对照组(P<0.001),电针组和氟桂利嗪组残存毛细胞计数显著高于模型组(P<0.001)。结论:电针可增强听觉神经系统传出性GABA的B受体表达,保护耳蜗形态,改善听觉功能。电针的效应优于氟桂利嗪。
Objective： To investigate electroacupunctrue （EA） adjusting the expression of the efferent neurotransmitter of gamma-aminobutyric acid B receptor （GABAsR） to affect the configuration and function of the organ of Corti in cochlea by comparing the effects of fltmarizine hydrochlorlde on vertebrobasilar insttfiqciency （VBI） rabbit models. Methods： Injected with sclerosant -775 injection into the soh tissue on the left side of cervical vertebral transverse processes of rabbits to set up cervical spondylotic vertebral arteriopathy model with VBI. The control group was injected with saline. Results： From the 6th weeks after injecting , acupuncture points at bilateral ＂Fengchi （GB 20） Tingkong （SI 19）＂ and ＂ Waikuan （SJ5）＂ were administered to the rabbits in the EA group , and the flunarizine group was given flunarizine hydrochloride by affusing stomach. After 2 weeks, the thresholds of cochlear nerve compound action potential （CAP） in the model group, EA group and fltmarizine group were higher than those in the control group （ P 〈 0. 001 ）, and the EA group and flunarizine group were lower than those in the model group （ P 〈 0. 001 ） . The thresholds of CAP induced by high frequency tone burst in the EA group and flunarizine group were higher than those induced by low frequency （ P 〈 O. 05） . The integral light density of GABAB R positive reactant in the model group was significantly lower than that in the control group （ P 〈 0. 001 ）, and those in the EA group and flunarizine group were evidently higher than that in the model group （ P 〈 0. 01 ）, and there was no difference among the three groups （ P 〉 0. 05 ） . The number of survived hair cells in the model group, EA group and flunarizine group were remarkably less than that in the control group （ P 〈 0. 001 ）, and those in the EA group and flunarizine group were evidently more than that in the model group （P 〈0. 001 ） . Conclusion： It was suggested that EA could increase the expression of B receptor for efferent GABA in auditory nerve system to protect the configuration of the cochlea and improve auditory function. The effects of EA are superior to flunarizine.
出处
《成都中医药大学学报》
2012年第2期21-24,91,共5页
Journal of Chengdu University of Traditional Chinese Medicine
基金
四川省教育厅自然科学基金资助项目(No.01LC11)
关键词
椎-基底动脉供血不足
电针
γ-氨基丁酸
耳蜗
听觉
Vertebrobasilar insufficiency
electroaeupunctrue
gamma- aminobutyrie acid
cochlea
hearing
作者简介
郑重，男；主任医师，医学博士；研究方向：神经电生理诊断及针灸、经颅磁刺激治疗机理。
