摘要
采用双抗体夹心酶联免疫吸附法测定不同剂量抑肽酶对心脏体外循环 (CPB)术中肿瘤坏死因子 α(TNF α)的含量。结果显示 :CBP术后 2 4h血浆TNF α含量 ,在全量抑肽酶组较对照组有明显降低 (P <0 .0 5) ;在单次剂量抑肽酶组较对照组无差异 (P <0 .0 5)。
Objective: This study evaluates the anti inflammatory activity of full dose and pump prime only(low dose) aprotinin by means of comparing tumor necrosis factor α(TNF α) levels of both groups after CPB. Methods: Twenty nine adults with rheumatic heart disease were randomized into three groups: (1)full dose aprotinin treated group(Group A, n=10); (2)pump prime only aprotinin treated group(Group B, n=10), and (3) control group(Group C, n=9). Plasma concentrations of TNF α were measured by enzyme linked immunosorbent assay technique at baseline(before operation), and at 2,24 hours after CPB termination. Results: A significant(P<0.05) increase of TNF α occurred in all three groups at 2 and 24 hours after CPB termination when compared with the same group at baseline. In Group A, TNF α level was significantly lower than that in group C(P<0.05) at 24 hours after CPB, but not in group B(P>0.05). Conclusions: Both CPB and operative stimulus induce the increase of cytokine TNF α after CPB. Full dose aprotinin has the anti inflammatory effect by means of reducing TNF α level after CPB. Low dose aprotinin dose not reduce TNF α level. So it no significant anti inflammatory effect. [
出处
《湖南医科大学学报》
CSCD
2000年第2期166-168,共3页
Bulletin of Hunan Medical University
关键词
抑肽酶
体外循环
TNFΑ
心脏外科手术
cardiopulmonary bypass
cytokine
systemic inflammatory response
aprotinin
