摘要
目的观察细菌内毒素脂多糖（LPS）刺激人内皮祖细胞（EPCs）后不同浓度血必净注射液对其增殖、黏附功能的影响。方法将EPCs经复苏、培养、传代、鉴定为正在分化的EPCs后，等量接种到培养板上，随机分为空白对照组[微血管内皮细胞培养液-2（EGM-2MV）]、LPS组（100μg／L）、血必净1组（LPS刺激EPCs后加入5、25、50g／L血必净注射液共同孵育）、血必净2组（LPS加5、25、50g／L血必净注射液同时孵育EPCs），应用四甲基偶氮唑盐（MTT）比色法测定EPCs的增殖和黏附能力[吸光度（A）值]。结果与空白对照组比较，LPS组EPCs增殖、黏附能力均显著降低（增殖能力：0．1077±0．0026比0．1248±0．0037，黏附能力：0．0647±0．0020比0．0770±0．0052，均P〈0．05）；与LPS组比较，不同浓度血必净组EPCs增殖能力均明显提高，且呈剂量-效应关系（均P〈0．05），EPCs黏附能力有所改善，但未见明显剂量一效应关系，以血必净1组25g／L浓度升高更显著（0．0855±0．0030，P〈0．05）。结论单纯LPS刺激可导致EPCs增殖、黏附能力下降；血必净注射液可使受损的EPCs增殖、黏附能力改善，其中增殖能力随血必净注射液剂量增加而增强，而黏附能力与血必净注射液剂量间未见明显关系。提示血必净注射液可不同程度中和LPS对EPCs的损伤，在革兰阴性杆菌所致脓毒症早期使用血必净注射液可能有更好的效果。
Objective To investigate the effect of Xuebijing injection at different concentrations on the proliferation and adhesion of endothelial progenitor cells （EPCs） obtained from human umbilical venous blood incubated by lipopolysaccharide （LPS） in vitro. Methods After being resuscitated, cultured and passaged, EPCs were assessed as differentiating cells, inoculated into the cultural plate, and divided equally into four groups ： control group [ cultivated with microvaseular endothelial growth medium-2 （EGM-2MV） fluidl, LPS group （LPS 100 ~tg/L） and Xuebijing 1st group （first incubated by LPS for 24 hours then added 5, 25, 50 g/L Xuebijing injection） and Xuebijing 2nd group （incubated with LPS and 5, 25, 50 g/L Xuebijing injection for 48 hours）. The influences of LPS and Xuebijing injection on EPCs＇ proliferation and adhesion （ absorbance （A） value] were evaluated by four methyl thiazolyl tetrazolium salt （MTT） assay. Results Compared with the control group, the proliferation and adhesion of EPCs in LPS group were declined significantly （the proliferation ： 0.1077±0.0026 vs. 0.1248±0.0037, the adhesion ： 0.0647±0.0020 vs. 0.0770±0.0052, both P〈0.05）. Compared with the LPS group, the proliferation of EPCs were improved when Xuebijing injection was added, presenting dose-effect relationship （all P〈0.05） ; the adhesion of EPCs was also improved but no dose-effect relationship was seen, and the most significant improvement was in the group where 25 g/L Xuebijing injection was added in the Xuebijing 1st group （0.0855±0.0030, P〈 0.05）. Conclusion LPS can decrease the proliferation and adhesion of EPCs in certain degree. Xuebijing injection can improve the proliferation and adhesion of injured EPCs, whose proliferation is increased along with the increase of the dosage of Xuebijing injection, while their adhesion has no such dose-effect relationship, suggesting that Xuebijing injection have certain degree of action to neutralize the EPCs＇ injury by LPS. Early application of this injection can make the treatment of sepsis induced by Gram-negative bacteria more effective.
出处
《中国中西医结合急救杂志》
CAS
北大核心
2012年第4期223-225,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
山东省中医药科技发展计划项目（200920）
关键词
血必净注射液
内皮祖细胞
增殖
黏附
中药注射液
Xuebijing injection
Endothelial progenitor cells
Proliferation
Adhesion
Traditional Chinesemedicine injection
作者简介
徐敏（1981-），女（汉族），山东省人，主治医师。
通信作者：孙运波，Email：sunyunb0163@163．com
