摘要
圆弧青霉PG3 7是碱性脂肪酶的高产菌 ,在摇瓶和 2 5L发酵罐小试的基础上进行 3M3发酵罐中试 .适合的发酵条件为 :发酵培养基 (% ) 豆饼粉 3 .0 ,玉米浆 3 .0 ,磷酸氢二钾 1 .0 ,硫酸镁0 .1 ,大豆磷脂 0 .5,柠檬酸钠 0 .0 5,花生油 0 .2 ;发酵起始 pH值为 8.0 ,发酵温度 2 9± 1℃ ,接种量8%～ 1 0 % ,通风量 0 .8L/ (L·min) ,搅拌转速 2 4 0r/min ,发酵周期 72h ,期间于 3 0、4 2、54h分别各流加 0 .4 %花生油 .在上述条件下 ,圆弧青霉PG3 7发酵酶活达 2 0 0 0 μmol/ (min·mL)
Penicillium cyclopium PG37 could produce high yield alkaline lipase, whose fermentation conditions in 3M 3 agitation fermentor were studied on the basis of agitation flask and 25 L fermentor tests. The experimental results showed that the optimal fermentation conditions were as follows, medium consisted of 3.0% soybean meal, 3.0% corn steep liquor, 1.0% K 2HPO 4, 0.1% MgSO 4, 0.5% soybean lecithin, 0.05% sodium citrate, and 0.2% peanut oil. The cultivation was carried out at the conditions of 29±1℃ with pH 8.0 in initial , 8%～10% inoculum size, 240 r/min agitation speed, and air volume 0.8 L/(L·min) for 72 h, during which 0.4% peanut oil was fed at 30, 42, and 54 hour respectively. With these conditions, the alkaline lipase yield by Penicillium cyclopium PG37 was about 2 000 μmol/(min·mL)
基金
国家"八五"科技攻关项目!资助课题 ( 85- 0 8- 0 3- 0 7)
关键词
碱性脂肪酶
圆弧青霉
发酵
酶制剂
alkaline lipase
Penicillium cyclopium
fermentation
