摘要
目的观察丝胶对2型糖尿病大鼠胰岛细胞凋亡的保护作用。方法雄性SD大鼠36随机分为3组:正常对照组、糖尿病模型组和丝胶治疗组。链脲佐菌素(STZ,25 mg/kg,连续3 d)腹腔注射建立2型糖尿病大鼠模型;待模型成功建立后,丝胶治疗组大鼠给予丝胶灌胃(2.4 g.kg-1.d-1)35 d。免疫组织化学染色观察胰岛β细胞Bcl-2和Bax蛋白的表达。结果与正常对照组大鼠相比,模型组大鼠胰岛β细胞Bax蛋白的表达明显升高(P<0.01),Bcl-2蛋白的表达明显降低(P<0.01);与模型大鼠相比,丝胶治疗组大鼠胰岛β细胞Bax蛋白的表达明显降低(P<0.01),Bcl-2蛋白的表达明显升高(P<0.01)。结论丝胶可通过上调胰岛β细胞Bcl-2蛋白的表达、下调胰岛β细胞Bax蛋白的表达,抑制2型糖尿病大鼠胰岛细胞凋亡,对糖尿病时胰岛细胞损伤具有一定的保护作用。
Objective To observe the protective effects of sericin on islet cells apoptosis of type 2 diabetes rats.Methods 36 male SD rats were randomly divided into 3 groups and each group had 12 rats： normal control,diabetes model and sericin treatment groups.Diabetes model were made by injecting 2% STZ（25 mg/kg,3 d） into the abdominal cavity continuously.After the diabetes model was successfully established,the rats in model group had no more treatment;the rats in sericin treatment group were lavaged with sericin（2.4 g·kg-1·d-1） for 35 days.SP immunohistochemical staining was used to observe the expression of Bcl-2 and Bax protein in β cells of islet.Results Compared with normal control rats,the Bcl-2 expression in β cells of islet of rats in model group was decreased obviously,Bax expression increased obviously（P0.01）.Compared with model rats,the Bcl-2 expression in β cells of islet of rats in sericin treatment was increased obviously,Bax expression decreased obviously（P0.01）.Conclusions Sericin can inhibit β cell apoptosis by up-regulating Bcl-2 expression and downregulating Bax expression and have protective effects on β cells injury during diabetes.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2012年第12期2525-2527,共3页
Chinese Journal of Gerontology
基金
河北省科技厅项目(No.08276101D-19)
河北省教育厅项目(No.2006301)
作者简介
刘晓燕（1965-），女，硕士，副主任医师，副教授，主要从事糖尿病神经病变临床研究。
通讯作者：陈志宏（1971-），女，博士，教授，硕士生导师，主要从事神经解剖学研究。
