摘要
目的:建立测定双黄祛毒片(盐酸小檗碱、大黄、五倍子)含量的方法。方法:采用HPLC在同一色谱条件ZORBAX SB-Pheny色谱柱(4.6 mm×150 mm,5μm)对没食子酸、盐酸小檗碱、大黄酸、大黄酚、芦荟大黄素、大黄素、大黄素甲醚进行含量测定。结果:线性范围分别为0.12～2.4μg(r=0.999 9),盐酸小檗碱线性范围0.11～2.2μg(r=0.999 9),0.015～0.3μg(r=0.999 8),0.02～0.4μg(r=0.999 8),0.008～0.16μg(r=0.999 9),0.013～0.26μg(r=0.999 8),0.006～0.12μg(r=0.999 9),平均回收率分别为100.97%,100.20%,99.01%,99.76%,101.10%,101.97%,101.56%。结论:该方法简便、快速、准确,重复性好,可作为双黄祛毒片中没食子酸、盐酸小檗碱、大黄酸、大黄酚、芦荟大黄素、大黄素、大黄素甲醚含量测定方法。
Objective： To establish a method for the determination of gallic acid, berberine hydrochloride, aloe emodin, rheiM, emodin, chrysophanol and physcion in Shuanghuangqudu tablets. Method： The HPLC system consisted of a ZORBAX SB-Pheny Dimensions column （4.6 mm × 15 cm, 5 μm） , methanol- 0.1% phosphoric acid as mobile phase （ using gradient） with flowing rate 1.0 mL ·min^-1 and detected at 310 nm. Result： The linear ranges of gallic acid, berberine hydrochloride, aloe emodin, rheiM, emodin, chrysophanol and physcion were 0.12-2.4 μg （r=0.9999）, 0.11-2.2 μg （r =0.9999）, 0.015-0.3 μg （r =0.999 8）, 0.02-0.4 μg （r = 0. 999 8）, 0. 008-0. 16 μg （r = 0. 999 9）, 0. 013-0.26 μg （r = 0. 999 8）, 0. 006-0.12 μg （r =0. 999 9）, respectively, and the average recoveries （ n = 9 ） were 100.97% , 100.20% , 99.01% , 99.76% , 101. 10% , 101.97% , 100.56% , respectively. Conclusion： The method is simple and repeatable, which can be applied to determine seven ingriedients at the same test condition in Shuanghuangqudu tablets.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第13期119-122,共4页
Chinese Journal of Experimental Traditional Medical Formulae
作者简介
陈云龙，研究生，从事中药新药研究，Tel：15920186407，E-mail：cyl_en@hotmail．com
[通讯作者]苏子仁，本科，研究员，从事中药新药研究。Tel：13763393599，E-mail：suziren@gzhtcm．edu．cn
