摘要
目的:探讨不同浓度人参皂苷Rg1、重组骨形态发生蛋白2(rhBMP-2)及两者联合应用对体外培养的人牙髓干细胞(DPSCs)成牙分化的影响。方法:酶消化法获得DPSCs,采用碱性磷酸酶(ALP)活性检测和实时荧光定量RT-PCR测定牙本质涎磷蛋白1(DSPP)和牙本质基质蛋白1(DMPl)mRNA的表达以检测不同浓度人参皂苷Rg1(0.1,0.5,2.5,5,10,20μmol/L)及rhBMP-2(25,50,100,200ng/ml)单独及联合应用对DP-SCs成牙分化的影响。结果:人参皂苷Rg1(2.5,5和10μmol/L)组的ALP活性明显高于同期对照组(P<0.001),5μmol/L组ALP活性明显高于其它浓度组(P<0.001);浓度为50,100,200ng/ml的rhBMP-2其ALP活性明显高于同期对照组(P<0.001),100ng/ml rhBMP-2组ALP活性最强。联合应用人参皂苷Rg1(5μmol/L)和rhBMP-2(100ng/ml)DSPP和DMP1 mRNA的表达明显高于单独应用组(P<0.001)。结论:人参皂苷Rg1可促进体外培养的DPSCs成牙本质分化,人参皂苷:Rg1协同rhBMP-2能明显增强DPSCs成牙本质分化。
Objective： The aim of the present study is to evaluate the effect of ginsenoside Rgl and recombinant bone morphogen-etic protein 2（rhBMP- 2） on the differentiation of human dental pulp stem cells （DPSCs）. Methods： DPSCs were isolated and cultured. The cells were incubated with different concentrations of ginseneside Rgl （ O. 1, 0.5, 2.5, 5, 10, 20/maol/L} and BaBMP - 2 [ 25, 50,100 和 200 ng/ml ）. The effect on the differentiation of DPSCs was assayed by ALP enzymatic activity, and the gene expression of dentin sialophesphoprotein（DSPP） and dentin matrix protein 1 （DMP1 ） by real time RT - PCR. Results： Comparing with the control group, ALP activity was sigaaificantly enhanced in ginseneside P, gl （2.5, 5, and 10μmol/L} group（P 〈 0. 001）, especially in ginsenoside Rgl （5tanol/L} group. ALP activity was significantly enhanced in rhBMP- 2 （ 50, 100,200 ng/ml ） group, especially in daBMP- 2 （100 ng/ml） group. The gene expression of DSPP and DMP1 were significantly increased in the combination group of glnsenoside Rgl （5μmol/L} and rhBMP- 2 （ 100 ng/ml ） compared with single application of ginsenoside Rgl or rhBMP - 2 only at the same time points （ P 〈 0.001）. Conclusion： The results indicate that ginsenoside Rgl promotes the differentiation of DPSCs. Combined application of ginsenoside Rgl and rhBMP2 can greatly promote DPSCs differentiation.
出处
《激光杂志》
CAS
CSCD
北大核心
2012年第3期89-91,共3页
Laser Journal
基金
重庆市科委自然科学基金项目(CSTC2011jjA10007)
重庆市卫生局项目(2011-2-056)
关键词
人参皂苷RG1
骨形态发生蛋白2
牙髓干细胞
分化
ginseneside Rgl
bone morphogenetic protein 2
dental pulp stem cells
differentiation
作者简介
王萍（1970-），女，重庆市人，副教授，博士，主要从事牙体牙髓、干细胞生物学研究。
通讯作者：王亚平。
