摘要
为揭示小麦自然群体干旱胁迫条件下旗叶叶绿素含量的变化,筛选相关标记的优异等位变异,以262份小麦种质资源组成的自然群体为材料,分别种植在北京的2个试验地点,均设雨养和灌溉处理,于开花期和灌浆期检测旗叶叶绿素含量。以分布于21条染色体的169个SSR标记检测所有材料的基因型,利用STRUCTURE2.3.2软件分析群体结构,用TASSEL软件的MLM(mixed linear model)方法对小麦自然群体的旗叶叶绿素含量进行关联分析。在此基础上,将携带某等位变异的所有材料表型均值与携带无效等位基因(null allele)材料表型均值比较,估计等位变异的表型效应,鉴别优异等位变异。共检测到2048个等位变异,每位点2～37个等位变异,平均12个。每位点的标记多态性信息量(PIC)为0.008～0.936,平均0.628。在22个标记位点共检测出40个(次)与旗叶叶绿素含量极显著的关联,其中11个标记位点有2次以上的关联,Xwmc419-1B和Xgwm501-2B分别有3次关联。在Xcfa2123-7A、Xgwm232-1D和Xgwm429-2B位点分别检测到效应值大于4.0的等位变异。
Elite alleles associated with chlorophyll content of flag leaf in a natural population of winter wheat （Triticum aestivum L.） consisting of 262 accessions were measured at flowering and grain-filling stages under both rainfed and well-watered conditions in two experimental locations in Beijing, China. A total of 169 SSR markers distributed on the 21 chromosomes of wheat were employed to detect the genetic diversity and genetic structure of the population. Association analysis between SSR loci and chlorophyll content trait was performed using TASSEL MLM （mixed linear model） program. The phenotypic allele effect was estimated through comparing the average phenotypic value over accessions with that of ＂null allele＂. A total of 2048 alleles were identified on the 169 SSR loci, and each locus had 2-37 alleles with an average of 12. The polymorphism information contents （PICs） of the SSR loci ranged from 0.008 to 0.936 with an average of 0.628. Forty markers were found to be significantly associated with chlorophyll content （P〈0.001） on 22 loci, of which 11 markers showed repeated associations, particularly, loci Xwmc419-1B and Xgwm501-2B had associations for three times. The alleles on loci Xcfa2123-7A, Xgwm232-1D, and Xgwm429-2B exhibited more than 4.0 of phenotypic effect value.
出处
《作物学报》
CAS
CSCD
北大核心
2012年第6期962-970,共9页
Acta Agronomica Sinica
基金
国家高技术研究发展计划(863计划)项目(2011AA100501)
国际农业研究磋商组织(CGIAR)挑战计划项目(G7010.02.01)资助
关键词
小麦
叶绿素含量
SSR
关联分析
等位变异
Wheat （Triticum aestivum L.）
Chlorophyll content
SSR
Association analysis
Allelic variation
作者简介
E-mail：li-wei-yu@126．com
李润植，E-mail：rli2001@hotmail．com
景蕊莲，E-mail：jingrl@caas．net．cn，Tel：010-82105829；
