摘要
目的 :对比地尔硫 (DTZ)及过氧岐化酶 (SOD)对心肌缺血再灌注损害的保护作用 ,以及明确联合应用 SOD+DTZ的疗效是否比单一药物更好。方法 :将 37只小型猪造成在体心肌缺血再灌注模型 ,分为 4组。 组 10只于再灌注前5分钟经静脉注射生理盐水作为对照组 , 组 9只再灌注前 5分钟静脉注射 SOD, 组 8只于再灌注前 5分钟静脉注射 DTZ, 组 9只于再灌注前 5分钟静脉注射 DTZ+SOD。结果 :(1)心梗范围 :用药各组与对照组比较 ,均有显著性缩小 ,DTZ+SOD组与 SOD组及 DTZ组比较 ,时性均有显著缩小 ;(2 )心肌钙离子含量 :SOD组与对照组比较有降低 ,但无显著性差异 ,而 DTZ组及 SOD+DTZ组与对照组比较均有显著的降低 ;(3) DTZ组及 SOD+DTZ组均可降低心率 ,减少再灌注性心律失常 ;(4)用药组均有降低灌注后 CK- MB的作用 ,但 SOD组的降低效果不明显 ,与对照组比较 ,无显著差异 ,而 DTZ组与对照组比较时性有显著性降低 ;SOD+DTZ组与 SOD组及 DTZ组比较均有显著性降低 ;(5 )电镜心肌超微结构观察显示 :心肌细胞损伤程度对照组比用药组严重 ,SOD组比 DTZ组及 SOD+DTZ组严重 ,DTZ组比 SOD+DTZ组严重。结论 :SOD、DTZ对缺血再灌注心肌有明确的保护作用 ,但 DTZ效果优于 SOD,合用 SOD+DTZ较应用其中单一药物效果更好。
Objective:To observe protective effect of Diltazem and superoxide dismutase on reperfusion injury of ischemic myocardium Methods:Thirty seven piglets were modelled as the reperfusion injury of ischemic myocardium The piglet's models were divided into 4 groups Group Ⅰ,10 peglets,were intravenously injected with physiological saline 10 minutes before reperfusion as controls Group Ⅱ,9 piglets were intravenously injected with SOD 5 minutes before reperfusion Group Ⅲ,8 piglets,were intravenously injected with DTZ 10 minutes before reperfusion Group Ⅳ,9 piglets,were intravenously injected with DTZ+SOD 10 minutes before reperfusion Results:The experimental result showed:(1)Myocardiac infarction size accounted for the percentage of weigtht of the left ventricle:In comparison between each drug therapeutic group and control group(23 47±2 34),the former were markedly reduced(SOD group 16 12±2 57 P<0 05;DTZ group 12 28±2 68 P<0 01;DTZ+SOD group 9 98±0 84 P<0 01;DTZ+SOD compared with SOD,the percentage of combined drugs therapeutic group was also markedly reduced,P<0 05 (2)Calcium ion content in myocardium:SOD group compared with control group SOD group is reduced,but there was no signigicant diffecence(control group 124 73±30 74μg/g,SOD group 110 06±21 15μg/g),while DTZ group(45 95±19 77μg/g) and SOD+DTZ group(70 72±25 27μg/g) compared with control group were markedly reduced,P<0 01 (3)In DTZ and SOD+DTZ group,the heart rate all were reduced,and the reperfusion arrhythmia were decreased too (4)The drug therapeutic groups all had the action of dropping CK MB after reperfusion,but in SOD group(3966 7±209 4u/L),its dropping effect was not significant,comparing with control group(4345 2±772 6u/L),it was not significant difference But DTZ(3561 2±133 7u/L),SOD+DTZ(3343 7±141 1u/L) group compared with control group,there were significant difference,P<0 01 Comparing with SOD,the DTZ and SOD+DTZ group were markedly reduced,P<0 01 Conclusion:There is markedly protective effect of SOD and DTZ on reperfusion injury of ischemic myocardium;but the efficacy of DTZ was better than SOD The efficacy of combined SOD and DTZ was better than just SOD or DTZ
出处
《广西医学》
CAS
2000年第1期3-6,共4页
Guangxi Medical Journal
基金
广西区科委 1996年科研项目
