摘要
目的:建立金钱草药材的HPLC指纹图谱,为金钱草药材的质控提供依据。方法:用水煎-正丁醇萃取-甲醇溶解制备样品溶液,采用RP-HPLC分析,色谱条件为Phenomenex C18色谱柱(4.6 mm×150 mm,5μm),以含0.5%冰醋酸的乙腈(A)-0.5%冰醋酸水(B)梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长255 nm,进样量20μL。利用中药色谱指纹图谱相似度评价系统(2004A版)对不同批次金钱草药材的HPLC图进行比较分析。结果:建立了金钱草药材的HPLC指纹图谱,以芦丁为参照峰,确立了金钱草药材指纹图谱中的13个共有峰。结论:所建立的指纹图谱稳定性和重现性均好,可用于金钱草药材的常规质量控制。
Objective： To establish the chromatographic fingerprint for Herba Lysimachiae by RP-HPLC.Methods： The sample solutions were prepared by extracting material with boiling water,followed by extracting with BuOH from the aqueous extract and analyzed by RP-HPLC with a Phenomenex C18 column（4.6 mm×150 mm,5 μm）,gradiently eluted with A（acetonitrile containing 0.5 % of acetic acid）-B（water containing 0.5 % of acetic acid） at 30 ℃,and monitored with UV detector at 255 nm.The flow rate was 1.0 mL/min,and sample volume injected was 20 μL.The chromatograms of different batches of Herba Lysimachiae were compared by the software of Similarity Evaluation System for Chromatographic Fingerprint of TCM（Version 2004A）.Results： HPLC Fingerprint of Herba Lysimachiae was established with Rutin as the reference compound.13 common peaks were selected as the fingerprint peaks of Herba Lysimachiae.Conclusion： The established HPLC fingerprint of Herba Lysimachiae has desirable precision,reproducibility,and can be applied to routine quality control of Herba Lysimachiae.
出处
《中药材》
CAS
CSCD
北大核心
2012年第3期382-385,共4页
Journal of Chinese Medicinal Materials
作者简介
郭林林（1986-），女，硕士研究生，专业方向：药用植物生物工程；Tel：13658351907，E-mail：happydaygoodluck1234@126．com。
通讯作者：邓君，Tel：023-68250761，Fax：023-68251225，E—mail：dengjcq@163．com。
