摘要
芜菁花叶病毒(Turnip mosaic virus,TuMV)危害范围广,变异快,对其序列进行分析,能对病毒的演变与进化有深入的了解,为抗病育种打下基础。P3蛋白是TuMV高度容易变异的蛋白之一,包含一个对Brassica属和Raphanus属不同宿主侵染能力的决定因子,决定了宿主范围。笔者分别从北京感病的萝卜和甘蓝上分离得到4个TuMV分离物BJ-R01和BJ-B01、BJ-B02、BJB03。利用RT-PCR克隆了这4个分离物的P3基因,测定了它们的核苷酸序列,并进行了序列分析。结果表明,4个分离物的P3基因均为1 065个碱基,编码355个氨基酸。4个分离物P3基因的同源性较高,核苷酸序列同源性在92.6%～99.3%,氨基酸同源性在93.5%～99.7%。根据系统进化树分析,4个TuMV分离物均属于world-B组。经对宿主的致病性鉴定,4个TuMV分离物均为BR致病型。4个TuMV分离物对Brassica属植物均表现出强致病性,但对Raphanus属植物致病性显现出明显的差异。
Turnip mosaic virus（TuMV） is a widely harmful and variable potyvirus.Analysis on the sequence of the virus is helpful to understand its evolution,and is the foundation for the virus resistance breeding.P3 protein is one of the most highly variable proteins of TuMV,which contains a key determinant of its ability to infect hosts in different genera,Brassica（B） and/or not Raphanus（R）.Four TuMV isolates（BJ-R01,BJ-B01,BJ-B02 and BJB03） were collected from the Raphanus and Brassica crops in Beijing,and the P3 gene were cloned from these isolates respectively.All the four P3 genes are 1 065 bp long,encoding 355 amino acids,and highly homologous.They share identities of 92.6%～99.3% and 93.5%～99.7% at nucleotide and amino acid respectively.The result of phylogenic analysis shows that these four isolates belong to TuMV world-B group.The pathogenic analysis reveals that all the four isolates belong to BR pathogenic type.They all have strong symptomatology to the Brassica,but show apparent difference to the Raphanus.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2012年第2期264-269,293,共7页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家高技术研究发展计划(863计划)项目(2008AA10Z154)
北京市自然科学基金(6092010)
作者简介
叶艳英（1986-），女，硕士生，主要从事蔬菜种质资源与分子育种，E-mail：yyy860925@126．com；
通讯作者：吴才君，教授，博士，E—mail：wucj12@126．com。
通讯作者：姚磊，副研究员，博士，E-mail：yaolei@baafs．net．cn
