摘要
建立了测定 O6-甲基鸟嘌呤浓度的高效毛细管区带电泳法。该方法以 p H9.0的硼砂为缓冲液 ,具有良好的重现性及线性关系 ,日内和日间变异系数分别为 1 .5 1 %和 1 .93 % ,平均回收率为 98.2 % ,相关系数为0 .9987,是一种简便、快速、准确、进样量少、灵敏度高的测定 O6-甲基鸟嘌呤的方法。
In this paper,a method to determine O6-methylguanine in urine by high performance capillary zone electrophoresis has been established. O6-methylguanine was directly introduced into the capillary employing sodium borate at pH 9.0 as buffer.The capillary used was 75 μm i.d.×57 cm . The calibration curve showed good linearity, r=0.998 7, CVs were 1.51% for intra-day and 1.93% for inter-day. The average recovery was 98.2%. In conclusion, this method is a simple, rapid, precise and reliable technique for O6-methylguanine measurement in urine.
出处
《色谱》
CAS
CSCD
北大核心
2000年第2期187-188,共2页
Chinese Journal of Chromatography
基金
国家卫生部资助基金! 96-1 -3 56
关键词
毛细管区带电泳
甲基鸟嘌呤
尿液分析
high performance capillary zone electrophoresis
O6-methylguanine
urine
