摘要
目的:观察1,2-二甲基肼(1,2-dimethy lhydrazine,DMH)是否可以诱导出小肠肿瘤,并与大肠进行比较.并探讨丁酸钠(sodium butyrate,NaBt)对DMH诱导肠道肿瘤的作用.方法:实验动物用SPF级♂Wistar大鼠,大小为8-9周龄,共分4组:DMH组、DMH+NaBt组、NaBt组、对照组.实验30-32wk之后过量麻醉使大鼠安乐死,取出小肠和大肠,观察肿瘤部位、数量、大小等;然后用10%甲醛固定,制作病理切片,观察各部位组织学改变.结果:实验结束时DMH组大鼠死亡率60.00%(18/30),DMH+NaBt组死亡率48.00%(12/25).DMH组肠道肿瘤发生率66.67%(8/12),4只肿瘤单发,4只多发,荷瘤率1.33(16/12),小肠肿瘤4个,大肠肿瘤12个;DMH+NaBt组肠道肿瘤发生率84.62%(11/13),6只为单发肿瘤,5只多发,荷瘤率1.46(19/13),小肠肿瘤3个,大肠肿瘤16个.两组之间肿瘤发生率及荷瘤率均无统计学差异.无论是DMH组还是DMH+NaBt组,结肠肿瘤发生率都高于小肠肿瘤,统计学有显著性差异(75.00%vs25.00%,P<0.05;84.21%vs15.79%,P<0.01).DMH组中肿瘤平均体积>0.05cm3个数占37.5%;DMH+NaBt组中肿瘤平均体积>0.05cm3个数占73.68%,两组肿瘤大小有统计学差异(37.50%vs73.68%,P<0.05).与DMH+NaBt组相比,DMH组浸润深度多局限于黏膜层内,有统计学差异(43.75%vs10.53%,P<0.05).结论:DMH也可诱导大鼠小肠肿瘤的发生,但发生率明显低于大肠肿瘤;NaBt可能促进肿瘤生长,关于NaBt对DMH诱导的肠道肿瘤作用仍需做进一步的深入研究.
AIM：To investigate whether 1,2-dimethylhydrazine （DMH） can induce small intestinal tumors in rats and to examine the effect of sodium butyrate on DMH-induced small intestinal and large intestinal tumors.METHODS：Eighty male Wistar rats were randomly divided into four groups：DMH group,DMH ＋ NaBt group,NaBt group,and control group.After 30-32 weeks,rats were euthanized with an overdose of intravenous pentobarbital （200 mg/kg）.After laparotomy,the small intestine and large intestine were dissected.The location,number,shape and size of intestinal tumors were examined and recorded.All tissues were subjected to hematoxylin and eosin （HE） staining to observe histological changes.RESULTS：The mortality rate of rats was 60.00% （18/30） in the DMH group and 48.00% （12/25） in the DMH ＋ NaBt group.The intestinal tumor incidence was 66.67% （8/12） in the DMH group with four small intestinal tumors and twelve large intestinal tumors observed.Four rats beared a single tumor while other four rats had multiple tumors.The mean number of beared tumors was 1.33.The intestinal tumor incidence was 84.62% （11/13） in the DMH ＋ NaBt group with three small intestinal tumors and sixty large intestinal tumors observed.Six rats beared a single tumor while other five rats had multiple tumors.The mean number of beared tumors was 1.46.There were no significant differences in tumor incidence and mean tumor number between te DMH ＋ NaBt group and DMH group.The large intestinal tumor incidence was significantly higher than the small intestinal tumor incidence in both the DMH group and DMH ＋ NaBt group （75.00% vs 25.00%,P 0.05;84.21% vs 15.79%,P 0.01）.There were significant differences in average tumor volume （37.50% vs 73.68%,P 0.05） and tumor infiltration depth （43.75% vs 10.53%,P 0.05） between the DMH group and DMH ＋ NaBt group.CONCLUSION：These results suggest that small intestinal tumors can also be induced by DMH.Sodium butyrate can increase tumor malignancy by increasing tumor volume and infiltration depth.
出处
《世界华人消化杂志》
CAS
北大核心
2012年第14期1184-1190,共7页
World Chinese Journal of Digestology
关键词
1
2-二甲基肼
丁酸钠
小肠肿瘤
大肠肿瘤
1
2-Dimethylhydrazine
Sodium butyrate
Small intestinal tumors
Large intestinal tumors
作者简介
通讯作者：王爱英，主任医师，100791，北京市海淀区花园北路49号．北京大学第三医院消化科．wangaiy@bjmueducn电话：010—82265533
