摘要
建立固相萃取-反相高效液相色谱法测定红毛丹中抑霉唑的残留动态分析的方法。色谱柱为Shim-pack VP-ODS(150mm×4.6mm,5μm),检测波长229nm,流动相为甲醇-离子对溶液(75:25,V/V),流速0.85mL/min,进样量20μL。抑霉唑在15.0～960.0μg/L(r=0.9994)范围内与峰面积呈良好线性关系,检出限为5μg/L,抑霉唑回收率为86.4%～104.5%,相对标准偏差0.84%～2.87%。同时,还对抑霉唑由红毛丹皮向肉的迁移,在红毛丹体上的降解动态进行分析。该方法操作简便快速,可作为红毛丹中抑霉唑含量监测的方法。
A reversed-phase high performance liquid chromatographic method with solid-phase extraction was proposed for the residue dynamic analysis of imazalil in rambutan.The analyte was separated on a Shim-pack VP-ODS（150 mm × 4.6 mm,5μm） using a mobile phased composed of a mixture at a volume ratio of 75：25 of methanol and acidic Na2H2PO4 solution approximately at pH 5（adjusted with 85% phosphoric acid） at a flow rate of 0.85 mL/min,and detected at 229 nm.The injection amount was 20 μL.An excellent linearity of imazalil in the range of 15.0-960.0 μg/L was achieved（r =0.9994）.The detection limit was 5.0 μg/L.The average spike recovery rates were in the range of 86.4%-104.5% with relative standard deviation of 0.84%-2.87%.The method was fast,simple,and applicable for the determination of imazalil residue in rambutan.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第8期188-190,共3页
Food Science
基金
贵州省遵义市科技局项目([2008]26号)
贵州省教育厅自然科学基金项目([2010]085号)
作者简介
高智席（1969-），男，副教授，硕士，研究方向为仪器分析。E—mail：wyhgzx@163．com
