摘要
目的 研究多巴胺 1(DA1)及多巴胺 2 (DA2 )受体激动剂对兔冠状动脉及肾动脉腺苷酸环化酶 (AC)活性的影响。方法 用放射免疫分析法测定cAMP含量 ,作为反映多巴胺受体功能的指标。结果 DA1受体激动剂非诺多泮(fenoldopam)及DA2 受体激动剂PBDA均可剂量依赖性增加冠状动脉及肾动脉cAMP的生成量。肾动脉cAMP的生成量均显著高于冠状动脉cAMP的生成量 ,选择性DA1受体阻断剂SCH2 3 390能够阻断fenoldopam及PBDA所引起的cAMP生成量的增加 ,而DA2 受体阻断剂domperidone则对PBDA的这一作用没有影响。结论 兔肾动脉及冠状动脉上都存在有剌激AC活性的DA1受体 ,但冠状动脉DA1受体的位点数比肾动脉DA1位点数要少得多 。
AIM To study the effects of DA 1 and DA 2 receptor agonists on rabbit coronary and renal arteriesMETHODS cAMP levels were measured by radioimmunoassay as an index of dopamine receptor functionRESULTS Both the selective DA 1 receptor agonist,fenoldopam,and the DA 2 receptor agonist,propgl propylbutyldopamine(PBDA),induced a doserelated increases in cAMP formation in coronary and renal arteries;however,the magnitude of increase in the renal artery was remarkably greater than that in the coronary arteryThe stimulatory effect on AC activity of fenoldopam was significantly more potent than that of PBDAThe selective DA 1 receptor antagonist, SCH23 390, blocked fenoldopam and PBDAinduced cAMP production,while the selective DA 2 receptor antagonist,domperidone,was without effect on the increase of cAMP elicited by PBDACONCLUSION The existence of DA 1 receptors associated with stimulation of AC activity in both renal and coronary arteriesHowever,there are much fewer receptor sites in the coronary artery than in the renal artery,suggesting less physiological importance of such receptors in the coronary artery than in the renal artery
出处
《中国药理学通报》
CSCD
北大核心
2000年第1期54-57,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助课题 !No39230150
关键词
非诺多泮
PBDA
冠状动脉
肾动脉
CAMP
cyclic AMP
coronary artery
renal artery
fenoldopam
SCH23 390
domperidone
