摘要
目的观察药典法定剂量关木通水煎剂对大鼠肾功能及肾间质结构的影响。方法用药典规定剂量的关木通水煎剂给大鼠灌胃（1g／kg，约相当于人类剂量0．1g／kg），每日1次，共两个月。期间观察各项肾功能指标、肾脏组织形态学改变及细胞外基质（纤连蛋白）分布情况。结果关木通给药组的血肌酐水平、尿酶（NAG和γ－GT）活性、尿蛋白、尿糖与正常组大鼠相比无统计学差异。关木通给药组大鼠的肾组织形态结构在光镜下未见明显异常改变，其纤连蛋白的分布与正常组完全相同。结论药典法定剂量的关木通水煎剂对大鼠肾功能及肾间质结构无明显的不利影响。
Objective To investigate the influence of caulis tristolochiae manshuriensis (CAM) decoction on renal function and interstitial structure in rats at statutory dose according to Chinese pharmacopoeia. Methods Wistar rats were given daily dose of 1g/kg (equivalent to 0. 1g/kg in human)CAM decoction for 2 months by gavage. The parameters of renal function and renal histology were examined.Distribution of extracellular matrix (fibronectin) was also examined by immunohistological method. In addition, large dose (60g/kg) of CAM decoction was given to Wistar ras for 4 days, and serum creatinine and renal histology were also exandned. Results Large dose of CAM decoction induced acute renal failure in Wistar rats, tubular damage was the underlying lesion. However, in rats given with CAM decoction at dose according to pharmacopoeia, there were no statistical differences in serum creatinine, urinary activities of N-acetyl-beta-glucosaminidase (NAG ) and gamma-glutamyl transpeptidase (γ-GT ), urinary excretion of protein and glucose between CAM group and control group. Histological examination did not reveal significant abnormality in the kidney of CAM group. The architectare of renal interstitium was intact, and the distribution of fibronectin was just as same as control group. Condusion There is no significant adverse effect of CAM decoction on renal function and interstitial structure in rats at statutory dose according to pharmacopoeia.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2000年第2期106-109,共4页
Chinese Journal of Nephrology
基金
卫生部临床学科重点项目!97020213
关键词
肾功能
肾间质
关木通
毒性实验
Renal failure
Renal interstitium
Caulis aristolochiae manshuriensis
