摘要
目的探讨慢性HBV感染者甘露糖结合蛋白（MBP）基因多态性对慢性乙型肝炎患者疾病进展的影响及与HBVDNA载量的关系。方法采用聚合酶链反应一限制性片段长度多态性（PCR—RFLP）方法和实时荧光定量PCR（FQ—PCR）技术对244例慢性乙型肝炎患者、151例肝硬化患者和88名正常对照者的MBP基因第54号密码子多态性和血清HBVDNA载量进行检测。结果CHB轻、中度组患者MBP基因GGC／GAC基因型频率和GAC等位基因频率与正常对照组比较差异无统计学意义（P〉0．05）；CHB重度组、代偿性LC组、失代偿性LC组MBP基因GGC／GAC基因型频率和GAC等位基因频率显著高于正常对照组（P〈0．05）；其中失代偿性LC组突变率最高，为36．5％。慢性HBV感染者MBP基因GGC／GAC基因型频率和GAC等位基因频率不随HBVDNA载量而变化（P〉0．05）。结论MBP基因第54号密码子突变与HBVDNA载量无明显关系，而与慢性HBV感染进展有关。
Objective To determine the influences of Mannose binding protein （MBP） gene polymorphisms on HBV DNA loads and on the progression of liver disease in patients with chronic HBV infection. Method The Codons on 54 MBP gene polymorphisms and HBV DNA loads in a cohort of 395 patients with chronic HBV infection, including 244 with chronic hepatitis B （CHB）, 151 with liver cirrhosis（LC） and 88 normal controls were examined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and fluorescent quantitative PCR （ FQ-PCR）. Result The MBP genotype frequencies of GGC/GAC and alleles genetic frequencies of GAC in CHB group showed no significant differences comparing to the normal control group （ P 〉 0.05 ）. The MBP genotype frequencies of GGC/GAC and alleles genetic frequencies of GAC on CHB group （severe） ,compensation phase of LC group and deeompensation phase of LC group were higher than those in the normal control group （P 〈 0.05 ） ,the genetic polymorphism of deeompensation of LC was 36.5% , highest of all. The MBP genotype frequencies of GGC/GAC and alleles genetic frequencies of GAC of patients with chronic HBV infection were not changed with the differences of HBV-DNA loads. Conclusion The codes on 54 MBP gene polymorphisms is not closely related to HBV DNA loads,but was associated with the progression of hepatitis B infection.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2012年第2期90-92,共3页
Chinese Journal of Experimental and Clinical Virology
基金
福建省漳州市科技计划资助项目（Z2010085）
作者简介
通信作者：高建平，Email：zhengruidan@tom．com
