摘要
目的建立凝胶色谱法测定美罗培南中聚合物的方法。方法采用SephadexG-10柱(10mm×300mm),流动相A为pH8.0的0.05mol/L磷酸盐缓冲液,流动相B为水。流速为0.3mL/min,检测波长为254nm,进样量为500μL,柱温为30℃,对照品采用法罗培南钠工作标准品。结果美罗培南在4.202～16.552mg/mL范围内与聚合物峰面积呈良好的线性关系(r=0.9999)。结论该方法准确、可靠、灵敏度高,适用于美罗培南聚合物的控制。
Objective To establish a gel filtration chromatography method for determination of the polymer in Meropenem.Methods The chromatographic column was Sephadex G-10（10 mm×300 mm）,mobile phase A,0.05 mol/L phosphate buffer（pH8.0）,mobile phase B for water;The flow rate was 0.3 mL/min,the detection wavelength was 254 nm and the injection volume was 500 μL,the column temperature was 30℃,reference standarda faropenem.Results The good linearity was obtained between peak area and concentration ranging from 4.202-16.552 mg/mL（r = 0.999 9）.Conclusion The method is simple,accurate and sensitive for the control of the polymer in Meropenem.
出处
《中国当代医药》
2012年第11期55-56,共2页
China Modern Medicine
关键词
美罗培南
聚合物
凝胶色谱法
测定
Meropenem
Polymer
Gel diltration chromatography
Determination
